首页> 中文期刊>中国实验血液学杂志 >保存期内红细胞内皮型一氧化氮合成酶mRNA表达的变化

保存期内红细胞内皮型一氧化氮合成酶mRNA表达的变化

摘要

This study was purposed to identify endothelial nitric oxide synthase (eNOS) mRNA in human RBCs during storage and to investigate the relationship of its changing profile and preservation time at 4℃. RT-PCR and gene sequencing were applied to identify eNOS-mRNA in banked RBC. Real time PCR was used to study the relationship of eNOS-mRNA expression and preservation time. The results showed that eNOS mRNA was detected in RBC. Compared with fresh RBC, the content of eNOS mRNA in RBC was 0.868 ±0.119 stored for 1 week, which was 0.379 ±0.289, 0.108 ±0.134, 0.141 ±0.141, 0.125 ±0.12 stored for 2, 3, 4 and 5 weeks respectively. It is concluded that eNOS mRNA exists in human RBC and its content is decreasing gradually along with the prolongation of storag time in banked RBC. Stored for 3 weeks, the content of eNOS-mRNA remains to be at lower level of concentration in human RBC.%本研究鉴定红细胞中有无内皮型一氧化氮合成酶(eNOS) mRNA,探讨在4℃保存条件下红细胞中的eNOS mRNA随保存时间变化的规律.采用逆转录聚合酶链反应(RT-PCR)和基因测序方法检测库存红细胞中有无eNOS mRNA的表达,采用实时荧光定量-聚合酶链反应(real time PCR)检测库存红细胞中eNOS mRNA的表达随时间变化的规律.结果表明:红细胞中eNOS mRNA检测结果呈阳性;保存1周的红细胞中eNOS mRNA含量为新鲜红细胞的0.868±0.119,保存2周为0.379±0.289,3周为0.108±0.134,4周为0.141 ±0.141,5周为0.125±0.12.结论:在红细胞中检测到eNOS mRNA;随着保存时间延长红细胞中eNOS mRNA含量逐渐下降,保存3周后eNOS mRNA表达量维持在一个低浓度水平.

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