mTOR (mammalian target of rapamycin) is the center for cellular activities. It controls many cell activities via inhibiting apoptosis and promoting cell growth. Rheb can activate mTOR signaling pathway and participate in genesis and development of multiple cancers. This study was purposed to explore the underlying role of Rheb in human myeloid leukemia by using the myeloid leukemia cell lines. Two myeloid leukemia cell lines HL-60 and K562 overex-pressing Rheb were established with retrovirus containing Rheb. The mRNA and protein expressions of Rheb were determined by Real-Time PCR and Western blot respectively. Cell proliferation rate was examined by CCK-8 assay and apoptosis rate was analyzed using Annexin V and 7-AAD double-staining. The results showed that Rheb was overexpressed in both HL-60 and K562 cell lines. The Rheb overexpression cell lines were successfully established. It is found that over-expression of Rheb could promote cell growth. Furthermore, the overexpression of Rheb could accelerate cells entering into G2/M phase (P <0.01), while did not affect the apoptosis. It is concluded that Rheb overexpression promotes myeloid leukemia cell proliferation through accelerating cell cycle progression.%mTOR信号通路是细胞内重要的生命活动枢纽,它通过抑制细胞凋亡,促进细胞增殖来调控细胞生命活动.Rheb可以激活mTOR信号通路,进而参与多种肿瘤的发生发展.本研究以髓系白血病细胞株为研究对象,探讨Rheb在HL-60和K562中的作用及相关机制.本研究利用逆转录病毒技术使髓系白血病细胞株HL-60和K562过表达Rheb;利用Western blot和Real-Time PCR分别检测HL-60和K562细胞中Rheb的蛋白表达水平及mRNA表达水平；利用CCK-8法检测细胞活力；利用Annexin V-PE和7-AAD双染检测细胞凋亡.结果表明:成功构建了Rheb过表达的HL-60和K562细胞株,并发现Rheb过表达可以促进细胞生长；进一步研究发现,Rheb过表达加快细胞进入G2/M期(P＜0.01),但不影响细胞凋亡.结论:Rheb通过加快细胞周期进程促进HL-60和K562细胞增殖.
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