Objective:To evaluate the correlation of single nucleotide polymorphisms (SNP) of Gemin 3 (rs197412) in the miRNA biosynthesis with NHL cancer risk and overall prognosis.Methods:miR-SNP were genotyped using PCR-ligase detection reaction (LAR,LCR) in NHL group of 230 non-Hodgkin lymphoma (NHL) patients and in control group of 120 healthy persons.The survival curves were drawn using the Kaplan-Meier method,and comparisons between the curves were made using the log-rank test.Multivariate survival analysis was performed by using a Cox proportional hazards model.Results:The rs197412 genotype distribution difference was not statistically significant,in NHL and control group;the survival time of patients carrying the rs197412 TT genotype was significantly longer than that of the patients carrying the CC + CT genotype (P =0.007).In addition,rs197412 was independent from the survival of NHL patients by multivariate analysis (RR:2.138,95 ％ CI:1.303-3.508,P ≤ 0.01).Conclusion:The single nucleotide polymorphisms of Gemin 3 (rs197412) in the miRNA processing are not related with NHL risk,but that may affect NHL survival.%目的:评估miRNA生物合成过程中Gemin3基因位点rs197412的单核苷酸多态性与患非霍奇金淋巴瘤(NHL)风险及预后的相关性.方法:在230名NHL患者组和120名健康对照者组中采用聚合酶链反应一连接酶检测反应进行MiR-SNP基因分型.利用Kaplan-Meier法计算患者生存曲线,曲线之间的比较采用对数秩检验.多因素生存分析采用Cox比例风险模型进行.结果:rs197412基因分型在NHL组及对照组中分布无差异.患者携带rs197412TT基因型比患者携带CC+ CT基因型生存时间长(P =0.007).此外,该rs197412 SNP也被确定为NHL预后的独立预测因子(相对危险度:2.138;95％可信区间:1.303-3.508;P=0.003).结论:miRNA生物合成通路中Gemin 3基因位点rs197412的单核苷酸多态性与患NHL风险无关,但可能影响NHL的生存.
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