健脾益气摄血方对辛伐他汀诱导斑马鱼出血模型的止血效果研究

摘要

目的:观察健脾益气摄血方对辛伐他汀诱导斑马鱼出血的止血效果并与清热凉血方比较.方法:用辛伐他汀0.5 μmol/L处理AB系斑马鱼24 h,建立斑马鱼出血模型,在严格盲态实验条件下,在最大非致死量范围内,应用不同浓度实验药物处理出血斑马鱼,以出血减少率、止血率为主要观察指标,以类似脾气虚特点的血流量、血流量改善率与运动速度、运动改善率为附加指标,综合评价健脾益气摄血方的干预效果.结果:实验药物B1在浓度为500和1 000 μg/ml时出血斑马鱼的出血率分别为30％和15％,止血率分别为60％和80％;实验药物B2在浓度为500和1 000 μg/ml时出血斑马鱼出血率分别为45％和40％,止血率分别为40％和47％.表明在降低斑马鱼出血率与止血效果两方面实验药物B1优于实验药物B2.在相等浓度下,在提高出血斑马鱼血流量与血流量改善率两方面实验药物B1优于实验药物B2.在相等浓度下,在促进斑马鱼运动速度与提高斑马鱼运动改善率方面实验药物B1优于实验药物B2.结论:健脾益气摄血方对辛伐他汀诱导的斑马鱼出血有良好的止血效果,并能够改善出血斑马鱼血流量与提高运动速度,为健脾益气摄血方治疗脾不统血证提供了实验依据.%Objective:To investigate the hemostatic effect of spleen-invigorating,qi-replenishing and blood-containing formula on simvastatin-induced zebrafish hemorrhage model,and to compare with the effect of clearing heat and cooling blood formula.Methods:Zebrafishes from breed A B line were treated with 0.5 μmoL/L simvastatin for 24 hours to establish zebrafishes hemorrhage model.Under strict blinded experimental conditions,the above mentioned zebrafishes were then treated with experimental drug of different concentrations at the maximum non-lethal dose.The intervention effect of spleen-invigorating,qi-replenishing and blood-containing formula was comprehensively assessed by examining the main observational parameters,such as bleeding reduction rate and hemostasis rate while referring to additional parameters,such as blood flow,improvement rate of blood flow,velocity of movement,improvement rate of motion,which are characteristics of spleen qi deficiency.Results:When the hemostatic effect of experimental drug B1 at the concentrations of 500 and 1 000 μg / ml,zebrafish bleeding rates were 30％ and 15％,the hemostatic rate was 60％ and 80％,respectively;when the experimental drug B2 at concentration of 500 and 1 000 μg / ml,Zebrafish bleeding rates were 45％ and 40％,the hemostatic rate was 40％ and 47％,respectively,showing that experimental drug B1 was superior to B2 in terms of decreasing bleeding rate and improving hemostatic effect in zebrafish.In the equal concentration,the experimental drug B1 was superior to B2 in terms of increasing and improving the blood flow of hemorrhagic zebrafish.Promotion and improvement of motion:in equal concentration,experimental drug B1 was superior to B2 in terms of promoting the motion velocity and increasing the improving rate of motion in zebrafish.Conclusion:The spleen-invigorating,qi-replenishing and blood-containing formula displays a good hemostatic effect on simvastatin-induced hemorrhage of zebrafish.It also boosts the blood flow and motion velocity in hemorrhagic zebrafish,therefore,providing an experimental basis for the treatment of syndrome of spleen failing to control blood by spleen-invigorating,qi-replenishing and blood-containing formula.