Degradation of crude oil by indigenous microorganisms supplemented with nutrients

摘要

Different kinds of mineral nutrients（NO3-N, NH4-N and PO4-P） were applied in the simulated oil-polluted seawater for enhancing oil biodegradation in the N/P ratio 10∶1 and 20:1 Although indigenous microorganisms have the ability to degrade oil, adding nutrients accelerated biodegradation rates significantly. For the group amended with NO3-N and PO4-P in the ratio 10∶1, the reaction rate coefficient was 4 times higher than the natural biodegradation. Chemical and microbiological analysis showed that the optimal N/P ratio in the system is 10∶1, and microorganisms tend to utilize nitrate rather than ammonium as N source.