以水华暴发的新鲜蓝藻为处理对象,采取冻融破壁的方式获取藻蓝蛋白的粗提液. 在一步盐析与二步盐析单因素试验的基础上,基于正交试验综合考虑盐析时间、藻蓝蛋白的浓度、pH和( NH4 ) 2 SO4 饱和度对纯化藻蓝蛋白的影响. 结果表明,一步盐析中最优工艺条件是,( NH4 ) 2 SO4 饱和度为17%、藻蓝蛋白粗提液浓度为1.45 g/L、pH为6.4、盐析时间为15 min;二步盐析中最优工艺条件是,(NH4)2SO4 饱和度为35%、pH为4.0、盐析时间为15min. 最终能得到纯度为2.04的藻蓝蛋白,有利于藻蓝蛋白的进一步纯化.%The fresh blue-green algae in algae bloom were used for treatment and the crude extracts of phycocyanin were obtained by freeze-thaw method.On the basis of single-factor experiment of salt precipitation in the first and second steps, salt precipitation time, the impacts of phycocyanin concentration, pH and ( NH4 ) 2 SO4 saturation on the purifying phycocyanin were comprehensively considered using the orthogonal experiment.The results showed that the optimum conditions in the first step were 17% of (NH4)2SO4 saturation, 1.45 g/L of crude phycocyanin extract, pH 6.4 and 15 min of reaction time.In the second step, the optimum (NH4)2SO4 saturation was 35%, with pH 4.0 and 15 min of reaction time.The purity of phycocyanin was 2.04 at last, which would facilitate further purification.
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