栀子对闹羊花急性中毒解毒效应的动物实验研究

摘要

目的 研究闹羊花的急性中毒现象以及栀子对闹羊花的急性解毒效应.方法 闹羊花的急性中毒实验,小鼠分别灌胃生理盐水和不同质量浓度的闹羊花水煎剂并对其行为学变化进行观察;栀子对闹羊花的急性解毒效应实验,小鼠分别灌胃生理盐水、闹羊花、栀子、闹羊花与不同质量浓度栀子的水煎剂,观察其形态学变化,检测心脏匀浆中的SOD活性、MDA含量与血清中CK含量.结果 闹羊花的急性毒性试验中,闹羊花的毒性表现为小鼠星醉酒状,翻正反射消失；小剂量时翻正反射能恢复,大剂量时小鼠死亡,且其中毒率、翻正反射消失率和死亡率随着剂量的增大而增高,而其翻正反射恢复率与剂量呈反比.栀子对闹羊花的解毒效应实验中,闹羊花组与生理盐水组和闹羊花+高剂量栀子组相比,CK含最明显偏高,且SOD活性较该两组偏低,MDA含量较生理盐水组和闹羊花+各剂量组偏高,但较栀子组明显偏低,并且闹羊花与栀子配伍小鼠情况更稳定.结论 闹羊花对小鼠具有神经系统和心血管系统毒性；栀子对闹羊花具有解毒效应,作用与两者配伍能减轻心脏的氧化损伤有关.%Objective: To study the acute toxicity of Flos Rhododendri Mollis and the detoxification of Fructus Gardeniae to it. Methods: Mice were administered intragastrically daily with the saline and several dosages of Flos Rhododendri Mollis. The change of behavior in these mice were observed. Meanwhile, mice were administered intragastrically with the Flos Rhododendri Mollis decoction, the Fructus Gardeniae decoction, and the Flos Rhododendri Mollis with different concentrations of Fructus Gardeniae. The SOD activity, the MDA content in heart and the serum CK were measured. Results: In the acute toxicity experiment of Flos Rhododendri Mollis, the toxicity was drunkness. The righting reflex was disappeared. When administration of low dosage, the righting reflex could be recovered. However, the mouse could be died when it was given the large dosage. The toxicity rate, the righting reflex disappear rate and the death rate were more and more higher as the decoction dosage was increased. However, the righting reflex had inverse ratio to its decociton dosage. In the Fructus Gardeniae to Flos Rhododendri Mollis experiment, the CK level of the Flos Rhododendri Mollis group was higher significantly than it of the saline group and the Flos Rhododendri Mollis plus high dosage Fructus Gardeniae group. However, the SOD activity in the Flos Rhododendri Mollis group was the least in 3 groups. Meanwhile, the MDA concentration in the Flos Rhododendri Mollis group was higher than it in the saline group and the Flos Rhododendri Mollis plus Fructus Gardeniae groups. But it was lower significantly than it in the Fructus Gardeniae group. The mouse condition was more stable in the combination of the Flos Rhododendri Mollis and the Fructus Gardeniae. Conclusion: Flos Rhododendri Mollis can induce neural and cardiovascular system toxicity on mice .Fructus Gardeniae has a detoxification effective on Flos Rhododendri Mollis via attenuate cordis oxidative damage.