电针对局灶性脑缺血再灌注大鼠血清白蛋白水平影响的实验研究

摘要

Objective:To observe the change of serum albumin level in MCAO rat's bloods by electro-acupuncture the points including Baihui (DU20),Shuigou (DU26),Zusanli (ST36) after cerebral ischemia/reperfusion (I/R).Methods:The MCAO rat's model was modified suture the brain artery.These changes were determined in serum albun in through BCG by Electro-acupuncture the pionts.Results:The level of serum albumin of normal control group were stability.Compared with the normal control group,the level of serum albumin of sham group rat was almost unchanged except the slight decrease in I/R24 h which was no significant difference.The level of serum albumin of model group and EA group showed a clear downward trend with particularly significant difference,in particular the model group compared with normal control group and sham group during the entire observation period.The level of serum albumin of EA group rat compared with model group showed remarakble differences.But the level of serum albumin of of EA group rat compared with normal control group and sham-operation group showed no differences.Conclusion:It is limited and insufficient to offset the declining trend that cerebral ischemia reperfusion and electro-acupuncture of endogenous improve the ability of serum albumin level of animal experiment.%目的 观察脑缺血/再灌注后,电针百会、水沟、足三里穴对局灶性脑缺血再灌注大鼠外周血清白蛋白浓度水平的影响.方法 采用改良线栓大鼠大脑中动脉法制作局灶性脑缺血再灌注模型,运用溴甲酚氯法,观察脑缺血/再灌注后大鼠外周血清白蛋白水平变化.结果 正常对照组大鼠血清白蛋白水平稳定；与正常对照组比较,假手术组大鼠血清白蛋白水平除在缺血/再灌注24 h时间点有轻度下降外,其余时间段无明显变化,与正常对照组十分接近；模型组和电针组大鼠血清白蛋白水平在整个观察时间窗内都呈现明显下降趋势,特别是模型组大鼠血清白蛋白水平,在各个时间点与正常对照组和假手术组比较,均具有非常显著差异；电针组大鼠血清白蛋白水平与模型组比较,在各个时间点均具有显著差异；电针组大鼠血清白蛋白水平在各个时间点与正常对照组、假手术组比较,均有明显差异.结论 脑缺血再灌注后电针内源性提高实验动物鼠血清白蛋白水平的能力十分有限,不足以抵消其明显的下降趋势.