参龙汤对脑缺血大鼠血清炎性因子的影响

摘要

Objective: To investigate the effect of Shenlongtang(SLT) decoction on the contents of tumor necro-sisfactor-α(TNF-α) and interleukin-6(IL-6)Jnterleukin-8(IL-8),intercellular adhesion molecule-1(ICAM-1) in blood serum of rats with cerebral ischemia model. Methods: The cerebral ischemia animal model was established by two-vessel occlusion.50 rats were randomly divided into 5 groups: control group with slam operation; model group;positive control group of pisacetam;SLT groups (low and high dosage). Four weeks later,The contents of TNF-α,IL-6,IL-8,ICAM-1 in blood serum were determined by enzyme linked immunosorbent assay (ELISA). Results: The contents of TNF-α,IL-6,IL-8,ICAM-1 in blood serum were significantly lower in the sham operation group than those in the cerebral ischemia group. The contents of TNF-α,IL-6,IL-8 and ICAM-1 in blood serum were significantly lower in the SLT high dosage group than those in the cerebral ischemia group. Conclusion: SLT has neuron protective effect by down regulation of the contents of the inflammatory cytokines in blood serum with cerebral ischemia rats.%目的 观察参龙汤对脑缺血大鼠血清炎性因子白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、细胞间黏附分子1(ICAM-1)、肿瘤坏死因子(TNF-α)的影响.方法 利用双侧颈总动脉结扎的方法制备慢性脑缺血模型,50只大鼠分为:对照组、模型组、脑复康组、大剂量参龙汤组及小剂量参龙汤组.药物处理4周后,采用ELISA法检测各组大鼠血清IL-6、IL-8、ICAM-1、TNF-α含量的变化.结果 脑缺血模型组大鼠的血清IL-6、IL-8、ICAM-1和TNF--α的含量较对照组显著升高.大剂量参龙汤组可显著降低血清中IL-6、IL-8、ICAM-1、TNF-α的含量,较模型组明显降低.结论 参龙汤大剂量组可显著降低血清中IL-6、IL-8、ICAM-1、TNF-α的含量,减少血清中炎性因子.这可能是参龙汤对脑缺血神经损伤的保护作用机制之一.