Objective:To study the protective effect of tanshinone Ⅱ A A sulfonate injection on coronary vascular endothelial injury in mice with viral myocarditis.Methods:60 Balb/c mice were selected,40 of them,with 3 consecutive days (d) intraperitoneal inoculation of Coxsackie virus B3 (CVB3 Nancy strain) method to establish a model of viral myocarditis in mice,after 10 d of viral myocarditis were randomly divided into model group(group VMC) and Danshen group(DST group),the other 20 as control(group BCG).In group DST,intravenous injection of tanshinone Ⅱ A sulfonate sodium injection was used to neat 7 d,group VMC and group BCG were injected with volume normal saline2.0 ml/(kg·d).From before and after the treatment of venous blood and heart in mice and isolated coronary arteries by ELISA test in mice sermn creatine kinase (CK),creatine kinase isoenzyme MB (CK-MB),lactate dehydrogenase (LDH),superoxide dismutase (SOD) and malondialdehyde (MDA).Immunohistochemistry was performed to detect the coronary artery of inducible nitric oxide synthase (iNOS) expression,determination of coronary vascular endothelial growth factor double antibody sandwich method (VEGF) expression,reverse transcription polymerase chain reaction (RT-PCR) detection of coronary artery VEGF-mRNA.Results:There was no significant difference between the VMC group and the DST group before treatment (P＞0.05).After treatment,serum CK,CK-MB,DST group LDH and MDA before treatment and VMC group decreased significantly,SOD increased significantly (P＜ 0.05);reduce coronary artery iNOS expression compared with that before treatment and VMC group was statistically significant (P＜ 0.05);and the high expression of VEGF mRNA and VEGF is higher than that of VMC group and the level of BCG group,compared with that before treatment,BCG group and VMC group were statistically significant (P＜ 0.05).Conclusion:Tanshinone Ⅱ A sulfonate injection can alleviate the damage of CVB3 and Nancy strains to the coronary vascular endothelium of Balb/c mice,and can protect the heart of the viral myocarditis model.%目的 观察丹参酮ⅡA磺酸钠注射液对病毒性心肌炎模型小鼠冠状动脉血管内皮损伤的保护作用并探讨其机制.方法 取Balb/c小鼠60只,选取其中40只,采用连续3d腹腔接种柯萨奇病毒B3 (CVB3Nancy株)的方法建立小鼠病毒性心肌炎模型,10d后随机分为病毒性心肌炎模型组和丹参组,另20只作空白对照.其中丹参组按2.0 mL/(kg·d)尾静脉注射丹参酮ⅡA磺酸钠注射液治疗7d,心肌炎模型组和空白对照组注射等体积0.9％氯化钠注射液.取治疗前后静脉血和小鼠心脏并分离出冠状动脉血管,采用酶联免疫吸附法检验小鼠血清肌酸激酶(CK)、肌酸激酶同工酶MB (CK-MB)、乳酸脱氢酶(LDH)、氧化物歧化酶(SOD)及丙二醛(MDA).采用免疫组化染色检测冠状动脉血管内皮诱导型一氧化氮合酶(iNOS)的表达,双抗体夹心法测定冠状动脉血管内皮因子(VEGF)表达,反转录-聚合酶链反应(RT-PCR)检测冠状动脉血管VEGF mRNA.结果 治疗后,丹参组血清CK、CK-MB、LDH及MDA较治疗前和心肌炎模型组明显降低、SOD明显提高(P＜0.05);冠状动脉iNOS阳性表达减少,与治疗前和心肌炎模型组比较差异有统计学意义(P＜0.05);VEGF及VEGF mRN高表达并高于心肌炎模型组和空白对照组,与治疗前、空白对照组和心肌炎模型组比较差异有统计学意义(P＜0.05).结论丹参酮ⅡA磺酸钠注射液可减轻CVB3 Nancy株对Balb/c小鼠冠状动脉血管内皮造成的损伤,对病毒性心肌炎模型心肌具有保护作用.
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