基于快速液相色谱技术对祛浊通痹复方质量控制的研究

摘要

Objective:To detect the levels of Astilbin,Diosgenin,Curcumin,Quercetin in Quzhuo Tongbi Fufang,and to establish its method of quality control.Methods:The content of Astilbin,Diosgenin,Curcumin and Quercetin in the formula were determined by the Rapid Performance Liquid Chromatographic (RPLC) technique.Results:The linear range of Astilbin,Diosgenin,Curcumin and Quercetin separately was 0.105～0.525 μg (R2=0.9993),0.637～3.185 μg (R2=0.9998),0.021～0.105 μg (R2=0.9999) and 0.0625～0.313 μg (R2=0.9977),respectively.The average plus sample recovery rate was 101.29％,97.35％,101.97％ and 99.18％,respectively.Conclusion:The method is accurate,reproducible and stable,and it can be used for quality control of Quzhuo Tongbi Fufang.%目的 测定祛浊通痹复方中落新妇苷、薯蓣皂苷元、姜黄素、槲皮素4种成分,建立祛浊通痹复方质量控制的方法.方法 采用快速液相色谱法(RPLC)对复方中的落新妇苷、薯蓣皂苷元、姜黄素、槲皮素进行含量测定.结果 落新妇苷、薯蓣皂苷元、姜黄素、槲皮素的线性范围分别为0.105～0.525 μg(R2=0.9993)、0.637～3.185μg (R2=0.9998)、0.021～0.105 μg (R2=0.9999)、0.0625～0.313 μg (R2=0.9977),平均加样回收率分别为101.29％、97.35％、101.97％、99.18％.结论 该方法准确,重现性和稳定性好,可用于祛浊通痹方的质量控制.