丹蒌片对动脉粥样硬化大鼠血管内皮功能及LOX-1表达的影响

摘要

目的 观察丹蒌片对动脉粥样硬化大鼠血管内皮损伤的保护作用以及作用机制.方法 40只雄性Wistar大鼠按照随机分为正常组、模型组、阿托伐他汀钙组(ATV组)、丹蒌片低剂量组(DLD组)、丹蒌片高剂量组(DLG组),每组8只.正常组大鼠喂食基础饲料,余4组均采用高脂饮食联合维生素D3(VD3)腹腔注射的方法复制大鼠动脉粥样硬化模型.模型组、ATV组、DLD组、DLG组分别给予0.9%氯化钠溶液、阿托伐他汀钙混悬液、丹蒌片低剂量、高剂量混悬液灌胃干预8周.干预结束后,检测大鼠血清中血脂、内皮素、NO和ox- LDL水平,Western- blot和RT- PCR法分别检测大鼠胸主动脉内LOX- 1蛋白和mRNA表达.结果 模型组大鼠血清中TC、TG、LDL- C和内皮素、ox- LDL、胸主动脉内LOX- 1水平较正常组升高,差异有统计学意义(P<0.05或0.01),NO水平较对照组下降(P<0.05或0.01).ATV组、DLD组、DLG组TC、TG、LDL- C和内皮素、ox- LDL、LOX- 1水平较模型组低,NO水平较模型组高,差异均有统计学意义(均P<0.05),ATV组与DLD、DLG组间比较差异无统计学意义(P>0.05).结论 丹蒌片可以改善动脉粥样硬化大鼠的血管内皮功能,可能与其降低血清ox- LDL水平,降低LOX- 1的表达有关.%Objective To investigate the effects of Danlou tablets on the vascular endothelial function and the expression of LOX- 1 in atherosclerotic rats.Methods Forty male Wistar rats were randomly and equally divided into five groups,namely control group,model group,atorvastatin group(ATV group),low- dose of Danlou group(DLD group),and high- dose of Danlou group (DLG group).Control group fed basic diet.Other four groups fed high fat diet plus intraperitoneal injection of vitamin D3 to create atherosclerosis model,and then received intragastric administration of saline,atorvastatin,low dose of Danlou tablets and high dose of Danlou tablets for 8 weeks,respectively.Serum lipid,ET,NO and ox- LDL levels were detected by ELISA method,the protein and gene expression of1ectiu- like oxidized low- density lipoprotein receptor- 1 (LOX- 1) in thoracic aorta were measured by Western- blot and real- time fluorescent quantitative PCR.Results Serum TC、TG、LDL- C、ET and ox- LDL levels,and LOX- 1 in thoracic aorta were significantly higher (P<0.05)and serum NO level was significantly lower (P<0.05)in model group than in control group (al P<0.05).Serum TC、TG、LDL- C、ET and ox- LDL levels (P<0.05)as well as LOX- 1 in thoracic aorta were significantly lower,and serum NO level was significantly higher in ATV group、DLD group and DLG group than in model group (all P<0.05).There was no significant difference of these parameters between ATV group and DLD group or DLG group.Conclusion Danlou tablets is effective in improving vascular endothelial function of athrosclerotic rats,which may be due to the decrease of serum ox- LDL level and LOX- 1 expression.