壬基酚胁迫对莱茵衣藻的生理影响

摘要

Effects of nonylphenol (NP) stress on growth, pigment content, activity of antioxidant enzymes and lipid per-oxidation of Chlamydomonas reinhardtii were studied. Results show that the growth of C. reinhardtii was inhibited by increasing NP concentration in the range of 0 - 0.8 mg · L-1 and after 5 days of incubation the inhibition rate in Treatment 0.8 mg · L-1 NP reached 84. 4%. The content of chlorophyll a increased first and then decreased but carotenoid content decreased gradually with rising NP concentration. In Treatment 0.6 mg · L-1 NP the content of chlorophyll a and the content of carotenoid was 16.7% and 37.2%, respectively, lower than that in the control(P < 0.05). The content of total soluble protein and malondialdehyde (MDA) decreased with increasing NP concentration, and was significantly lower in Treatment 0.4 and 0.6 mg · L-1 NP than in the control(P <0.05). Superoxide dismutase (SOD) and catalase (CAT) activity increased with increasing NP concentration and was 25.0 and 32.1 times higher in Treatment 0.6 mg · L-1 NP than in the control (P < 0.001), respectively. The content of glutathione (GSH) also increased with increasing NP concentration in the range of 0 -0.2 mg · L-1 and began to turn downwards when NP concentration rose beyond 0.2 mg · L-1, indicating that NP stress may induce rise of GSH content.%研究了不同浓度壬基酚(nonylphenol)对莱茵衣藻(Chlamydormonas reinhardtii)生长、色素含量、抗氧化酶活性和脂质过氧化作用的影响.结果表明,随着ρ(壬基酚)(0～0.8 mg·L-1)的升高,莱茵衣藻的生长受到抑制,培养5d时0.8mg·L-1壬基酚处理组抑制率达到84.4％.叶绿素a含量随着壬基酚浓度的升高而呈先升高后降低的变化趋势,类胡萝卜素含量则逐渐降低,0.6 mg·L-1壬基酚处理组叶绿素a和类胡萝卜素含量分别比对照组下降16.7％和37.2％(P＜0.05)；总可溶性蛋白和丙二醛(MDA)含量随壬基酚浓度的升高而呈下降趋势,0.4和0.6 mg·L-1壬基酚处理组这两个指标与对照组间差异均达显著水平(P＜0.05).超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性随壬基酚浓度的增加而不断上升,至ρ(壬基酚)为0.6 mg·L 1时SOD和CAT活性极显著升高(P＜0.001),分别为对照组的25.0倍和32.1倍.还原型谷胱甘肽(GSH)含量在一定浓度范围(0～0.2 mg·L-1)内随壬基酚浓度的升高而升高,当ρ(壬基酚)＞0.2mg·L-1时则下降,表明壬基酚胁迫可诱导GSH含量增加.