The influence of feeder layer, conditioned medium and differentiation inhibitory activity on isolation and clone of embryonic stem cell were introduced and the physiological mechanism for differentiation inhibitory activity influencing on isolation and embryonic stem cell in mammal animals was discussed in this paper .%介绍了饲养层、条件培养基、分化抑制因子对哺乳动物胚胎干细胞分离与克隆的影响,并探讨了白血病抑制因子影响胚胎干细胞克隆的机理。
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