采用布氏田鼠建立动物流感病毒感染模型的试验研究

摘要

In this study,the brandti voles were infected by influenza virus A/WSN/33(H1N1) intranasally,in order to assess the susceptibility of influenza virus on brandti voles.Based on the susceptibility,further research on the possibility of establising the animal model infected by influenza virus and its evaluation indexes were conducted.By infecting brandti voles,influenza virus was separated and identified successfully.The result showed that the virus was widely distributed in brandit vole tissues,which also indicated that the brandti vole are highly sensitive to influenza virus,and the major target organ was lung.The animal model of brandti voles infected by influenza virus has been established successfully by means of some model indexes,such as clinical symptoms,body weight,mortality,the index of the separation of influenza virus and so on.%应用流感病毒A/WSN/33(H1N1),通过人工滴鼻接种的方式感染布氏田鼠,评估其对流感病毒的易感性,并在其易感性较强的基础上,进一步对建立流感病毒感染布氏田鼠疾病动物模型的可能性及其评价指标进行研究.通过对感染的布氏田鼠进行体内病毒分离及鉴定等方法,成功分离到了流感病毒,证实了流感病毒在布氏田鼠组织中的存在.试验结果表明,流感病毒对布氏田鼠具有很强的感染性,其主要侵害的靶器官为肺脏.综合感染布氏田鼠的临床症状及病理变化、体质量变化、死亡率、流感病毒的分离及鉴定等指标,表明成功建立了流感病毒感染布氏田鼠的疾病动物模型.