The collagen-chitosan composite scaffolds were fabricated by lyophilization technique, which were structurally modified characteristics were determinated. by laminin （LN） protein, and their physical performance Neural stem cells （NSCs） obtained from the fetal mouse hippocampus were cultured in the collagen-chitosan composite scaffolds and the biocompatibility was studied. The comparative results of different scaffolds with the parameters of aperture, porosity, water absorption and degradation rate show that the volume ratio of 7 ： 3 is more suitable for cell culture in vitro and the inoculation rate of NSCs is markedly improved in LN-treated scaffolds. Through the observation of laser scanning confocal microscope （LSCM） and scanning electron microscopy （SEM）, NSCs within collagen-chitosan composite scaffolds present good growth, proliferation and differentiation. These results provide a scientific experimental basis not only for further NSCs clinical transplantation applications, but also for the drug screening and mechanism research on the treatment of diseases of the nervous system.%采用冷冻干燥法制备了胶原-壳聚糖复合支架并通过层粘连蛋白（LN）进行结构修饰，测定其物理性能特征，建立了胎鼠海马神经干细胞（NSCs）的三维培养体系，同时考察了NSCs与胶原-壳聚糖复合支架的生物相容性．孔径、孔隙率、吸水率、降解率等参数的比较表明，体积比为7：3的复合支架更适合于体外细胞培养的要求，并且NSCs在经LN修饰后支架材料上的接种率得到明显提高；激光共聚焦显微镜及扫描电镜观察表明，NSCs能够在胶原-壳聚糖复合支架内良好地生长、增殖及分化．这些结果为NSCs的进一步临床移植应用，以及治疗神经系统疾病的新药筛选和机理研究等奠定了坚实的实验基础．
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