基于COⅠ序列的不同地理种群岩虫遗传多样性研究

摘要

The genetic diversity and population genetic structure were investigated in 57 individuals in five geo-graphic populations of rock worm Marphysa sanguinea collected from Dalian,Xingcheng,Rushan and Beihai by a 440 bp segment of the mitochondrial DNA COⅠgene. In total,there were 78 polymorphic sites with 19 single vari-able sites,59 parsimony informative sites and nine haplotypes. The overall haplotype diversity and nucleotide diver-sity were 0.830 and 0.097 73 in the five populations, respectively, with the maximal genetic diversity in Rushan population and the minimal one in Dalian population. A neighbor-joining phylogenetic tree and a median-joining network revealed that most haplotypes were highly interconnected and all haplotypes clustered into two branches, displaying clear different geographical distributions in the studied area. The findings will provide useful information on biodiversity protection and artificial breeding of this species.%为揭示中国沿海岩虫Marphysa sanguinea自然群体的遗传多样性,采用线粒体COⅠ序列分子标记方法,对大连、兴城、乳山和北海4个海域共5个群体57个岩虫的遗传多样性及群体遗传结构进行了分析研究.结果表明:57个个体中共检测到78个多态性位点,其中简约信息位点59个,单核苷酸突变位点19个,共出现了9种单倍型;5个群体的单倍型多样性和核苷酸多样性分别为0.830和0.09773,整体的遗传多样性水平较高;5个群体中遗传多样性最高为乳山群体,最低为大连群体,岩虫5个群体聚为两大类.本研究结果为进一步开展岩虫的人工扩繁及资源保护奠定了理论基础.