Objective To make sure the prognostic relevance of TYMS for patients with pros-tate cancer (PCa)and lay the foundation for the early diagnosis and target therapy for PCa. Meth-ods PCa gene expression studies GSE32448,GSE16560,GSE17951 and GSE79957 were analyzed to make sure the difference of the expression of TYMS between PCa and normal prostate tissue,to determine the correlation between the expression of TYMS and the clinical features (age,TNM stag-ing,Gleason score,and serum PSA level)of PCa patients,and to investigate potentially relevant mechanisms. Results TYMS was significantly increased in PCa cells compared with normal pros-tate tissues (P=0.044 8).PCa patients in the TYMS low expression group had better T staging,Gl-eason score compared with PCa patients in the TYMS high expression group (P=0.002 and P<0.000 1,respectively).The overall survival favored patients with lower expression of TYMS com-pared with those with higher expression of TYMS.TYMS might promote the growth of PCa cells through several biological processes and signaling pathways including E2F signaling,MYC signaling, mitotic spindle,DNA repair,G2M checkpoint,and glycolysis. Conclusions TYMS predicted poor clinical features of patients with PCa and might be an independent risk factor for patients with PCa.%目的 探讨胸苷酸合成酶(TYMS)基因在前列腺癌(PCa)患者中的表达水平及其临床预后意义,为PCa的早期诊断和靶向治疗奠定基础.方法 通过在 GEO(gene expression om-nibus)数据库下载并分析 PCa 基因表达数据集 GSE32448、GSE16560、GSE17951以及 GSE79957,明确TYMS基因在PCa和正常前列腺组织中的表达差异,明确 TYMS基因表达与 PCa患者年龄、分期、Gleason评分以及总生存期的相关性,并探讨TYMS基因对于 PCa 细胞可能的作用机制.结果 TYMS在PCa中的表达水平明显高于其在正常前列腺组织中的表达水平(P=0.0448),高表达TYMS的PCa患者T分期、Gleason评分明显差于低表达 TYMS基因的 PCa 患者(P 值分别为0.002和<0.0001).低表达 TYMS 基因的 PCa 患者总生存期明显优于高表达 TYMS 基因的PCa患者(P=0.0056).基于 TYMS基因集富集分析表明,TYMS 可能通过多种信号通路(E2F、MYC)、细胞周期(有丝分裂、G2M检查点)以及细胞代谢(糖酵解)等途径影响PCa细胞增殖.结论 TYMS与PCa患者的不良预后密切相关,可作为PCa的独立危险因素.
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