Objective To explore optimal temperature of trehalose and glucose loading red blood cells for lyophiliza-tion, red blood cells were incubated with trehalose and glucose at different temperature in the present study. Methods The red blood cells were loaded with 0, 0.125, 0. 25. 0. 5 and 1 mol/L trehalose and glucose respectively in 4℃ , 25℃ and 37℃ for 6 hours. Then, the levels of free hemoglobin and lactate dehydrogenase in the supernatants were determined. Results It showed that free hemoglobin and lactate dehydrogenase significantly increased in three groups of 1 mol/L trehalose and glucose(4℃. 25'C and 37℃ ). In the trehalose and glucose treatment groups (0.125, 0. 25, and 0. 5 mol/L) at 25℃, the red blood cells showed significant hemolysis. However,red blood cells were loading with 0.125, 0. 25, and 0. 5 mol/L trehalose and glucose at 4℃ and 37℃, the free hemoglobin and lactate dehydrogenase were slightly increased. Conclusion These results suggested that loading with trehalose and glucose (0. 125, 0. 25, and 0. 5 mol/L) at 4℃ and 37℃ have little hemolytic effect on red blood cells and be qualified for loading red blood cells for purpose of lyphilization.%目的 探索海藻糖和葡萄糖联合负载红细胞的最适温度,为冷冻干燥红细胞提供基础.方法 分别采用0、0.125、0.25、0.5和1 mol/L的海藻糖联合葡萄糖在4℃、25℃和37℃负载红细胞6h.然后检测上清液中游离血红蛋白和乳酸脱氢酶含量.结果 负载液浓度为1 mol/L时,3种温度下的细胞溶血程度较重,即上清中游离血红蛋白和乳酸脱氢酶浓度较高.在浓度低于1 mol/L时,25℃负载后,红细胞溶血程度较高.4℃和37℃负载时,上清中游离血红蛋白和乳酸脱氢酶浓度较低.结论 在浓度小于1 mol/L时,海藻糖联合葡萄糖在4℃和37℃情况下,负载红细胞后对细胞的损伤较小,能够满足冻存的负载要求.
展开▼
