脑胶质瘤细胞P-糖蛋白表达及临床意义

摘要

Objective To explore the expression and clinical value ofP-glycoprotein in human glioma. Methods The paraffin embedded sections from 63 cases of human glioma were examined for expression of multidrug resistant P-glycoprotein ,by means of immunohistochemical staining with the JSB-1 antibody. Results P-gp-positive expression was observed in 25(39.68%) of 63 human glioma, and in 7(87.5%) of 8 recurrent glioma patients after chemotherapy(P<0. 005).We also investigated correlation between the expression of P-glycoprotein and the tumor grade. The positive rate of P-gp in glioma cells was 25% in grade Ⅱ, 38.46% in grade Ⅲ, 52.39% in grade Ⅳ respectively(P<0.005). Conclusions These results suggest that P-gp in human glioma may be acquired or primary.The positive rate of P-gp in glioma cells drug resistance increased after chemotherapy.Expression of P-gp was also associated with histologic grade of glioma.It can provide reliable evidence for the postoperative chemotherapy.%目的 探讨脑胶质瘤细胞P－糖蛋白(P-gp)的表达及临床价值。方法 采用s－p免疫组织化学法，对63例手术后脑胶质瘤标本石蜡切片，测定P-gp表达。结果 63例脑胶质瘤P-gp阳性率39.68%(25/63)；8例化疗后复发脑胶质瘤P-gp阳性率87.50%(7/8)；两者间存在显著差异（P<0.025）。63例脑胶质瘤病理分级中，P-gp阳性率Ⅱ级25.00%，Ⅲ级38.46%，Ⅳ级52.39%，病理分级与P-gp表达呈正相关（P<0.005）。结论 P-gp表达可为原发性和获得性的，化疗能诱导或增强肿瘤耐药性，P-gp表达随脑胶质瘤恶性程度增强，可作为临床化疗中的重要指导性指标。