血清IL-1β和IL-6表达水平与膝骨关节炎病程关系的研究

摘要

Objective To investigate whether innate differences in serum interleukin(IL) -1β and IL - 6 abundance are associated with the severity of knee osteoarthritis.Methods In this case - control study, blood samples from 85 patients with osteoarthritis and 85 age - matched controls were selected.According to the severity of osteoarthritis assessed by radiography followed by Kellgren/Lawrence (K/L)grade,patients were classified into low- grade(28 cases) ,medial- grade(37 cases)and high- grade(20 cases).Innate serum abundances of IL - 1β and IL - 6 were measured by ELISA.Their correlations with the severity of knee osteoarthritis were determined by the conditional Logistic regression and T - test.Result Blood IL - 1β abundance in low - grade, medial - grade and high - grade patients with osteoarthritis was 6.95 ± 3.75,8.63 ± 3.30, and 13.53 ± 9.23 pg/mL separately, which was all significantly higher than controls (4.08 ± 1.84 pg/mL).Similarly, blood IL - 6 abundance in low - grade, medial - grade and high -grade patients with osteoarthritis was 41.72 ± 18.29,54.41 ±26.14,and 74.28 ± 18.26 pg/mL,respectively, which was also significantly higher than controls( 25.04 ± 10.39 pg/mL).Moreover, increased severity of knee osteoarthritis was associated with increased innate production of IL - 1β and IL - 6.Analysis by conditional Logistic regression revealed that both IL - 1β ( OR = 1.32) and IL - 6 ( OR = 1.07 ) were risk factors of development of knee osteoarthritis ( P ＜ 0.01 ).Conclusion Blood IL - 1βand IL - 6 abundance could be used as novel biomarkers to access the severity and prognosis of knee osteoarthritis; Study on IL - 1β and IL - 6 would help illustrate the pathogenesis of knee osteoarthritis and provide new methods for clinical diagnosis and treatment.%目的 探讨膝骨关节炎患者血清IL-1β和IL-6表达水平与病程发展的关系.方法 85例骨关节炎患者,用膝关节X射线检测和Kellgren/Lawrence(K/L)评分法则分为轻度(28例)、中度(37例)和重度(20例)组,同时选取年龄性别匹配的85例健康人作为正常对照,用双抗体夹心ELISA法测定各组血液中IL-1β和IL-6的表达水平,T-TEST比较各组间差异,Logistic回归分析IL-1β和IL-6表达水平与关节炎病程发展的相关性.结果 轻、中、重三组患者血清中IL-1β的浓度分别为(6.95±3.75)pg/ml、(8.63±3.30)pg/ml、(13.53±9.23)pg/ml,明显高于对照组(4.08±1.84)pg/ml,表达水平随着病情分级逐渐加重而呈现出逐渐增高的趋势(P<0.01);同时,轻、中、重三组患者血清中IL-6的表达量分别为(41.72±18.29)pg/ml、(54.41±26.14)pg/ml、(74.28±18.26)pg/ml,明显高于对照组(25.04±10.39)pg/ml,表达水平随病程加重而逐渐增高(P<0.01).回归分析表明,IL-1β和IL-6都是膝骨关节炎病程发展的危险因素(OR值分别为1.32和1.07,P<0.001).结论 IL-1β和IL-6表达水平可作为判断膝骨关节炎临床病情和预后的免疫学指标;对IL-1β和IL-6的研究将有助于进一步在分子水平阐明膝骨关节炎的发病机制,为临床诊断和治疗提供新的思路.