分泌性白细胞蛋白酶抑制剂在胃溃疡愈合中的作用机制探讨

摘要

[Objective]To explore the role of secretary leukocyte protease inhibitor(SLPI)in the mechanism of peptic ulcer via investigating its expression and distribution in gastric mucosa during gastric ulcer healing.[Meth-ods]Expression of SLPI in gastric mucosa of 30 patients with gastric ulcer before and after treatment was exam-ined by ABC immunohistochemistry stain and reverse transcription polymerase chain reaction (RT-PCR).[Re-sults]The expressions of SLPI stain were found mainly in the cytoplasm of epithelial cells in gastric mucosa.Ab-sorbance of SPLI in gastric mucosa before treatment (A=0.7083±0.0593)was significantly higher than in the healing mucosa after treatment (A=0.2641±0.0236,P <0.05).Meanwhile,mRNA level change of SLPI by PT-PCR demonstrated very similar findings compared to that of SLPI protein expression change(P <0.05).[Conclu-sion]The overexpression of SLPI may be involved in gastric ulcer healing by regulating connective tissue format-ting and angiogenesis.%[目的]探讨分泌性白细胞蛋白酶抑制剂(secretory leucocyte protease inhibitor,SLPI)在胃溃疡愈合中的作用机制.[方法]采用免疫组织化学染色法、反转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)等方法,对30例胃溃疡患者愈合前后胃黏膜组织中SLPI的表达进行检测.[结果]免疫组化染色法显示,SLPI主要表达于胃黏膜上皮及胃腺上皮细胞胞质中,溃疡愈合前胃黏膜组织中SLPI的表达水平为A=0.7083±0.0593,与愈合后A=0.2641±0.0236相比明显增强(P<0.05)(A为平均吸光度).RT-PCR检测结果显示SLPI mR-NA的表达与其蛋白质的改变相一致(P<0.05).[结论]SLPI可能通过促进成纤维细胞和血管内皮细胞的增殖活化,促进结缔组织增生和微血管形成,从而促进溃疡愈合.