Objective To investigate mycobacterium tuberculosis antigen specific IFN-r response(Elispot) in patients with tuberculous meningeencephalitis or Ⅲ type pulmonary turberculesis. Methods 383 cases of tuberculosis patients ( including 73 cases of tuberculous meningeencephalitis and 310 cases of Ⅲ type pulmonary turberculesis ) and 97 bealthy volunteers were enrolled in our study. The antigen specific IFN-r responses were evaluated by Elispot assay. Results The sensibility of Elispot in tuberculosis patients is 81.64％,and the specificity is 85.57％. Positive IFN-r response were detected in 61.64％ tuberculous meningeencephalitis group, 86.13％ in Ⅲ type pulmonary turbercuiosis group and 10.31％ in healthy wolunteers. There were statistical differences between groups( P ＜ 0. 05 ).Conclusion Antigen-specific IFN-r Elispot assay could be a favourable assistant method in the diagnosis of Tuberculosis. It has better value in Ⅲ type pulmonary tuberculosis than in tuberculous meningeencephalitis.%目的 评价酶联免疫斑点技术(Elispot)检测外周血结核杆菌抗原特异性r干扰素(IFN-r)水平在结核脑膜脑炎及Ⅲ型肺结核患者诊断中的应用价值.方法 利用Elispot技术分别对383例结核病人(包括73例结核性脑膜炎患者、310例Ⅲ型肺结核患者)、97例健康人外周血结核杆菌抗原特异性r干扰素(IFN-r)水平进行定量检测.结果 Elispot技术在结核病中诊断敏感性为81.46%,特异性为85.57%;结核脑膜脑炎组Elispot阳性率为61.64%;Ⅲ型肺结核患者组Elispot阳性率为86.13%;健康对照组Elispot阳性率为10.31%;各组间比较差异具有统计学意义(P＜0.05).结论 Elispot技术在结核病诊断中具有良好的辅助诊断价值,在Ⅲ型肺结核中的诊断价值优于结核性脑膜脑炎.
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