γ-干扰素释放分析T-SPOT.TB在结核性疾病中的诊断价值

摘要

目的 评价γ-干扰素释放分析T-SPOT.TB检测在结核性疾病中的诊断价值.方法 采用T-SPOT.TB试剂盒对疑诊或待排结核患者外周血中释放γ-干扰素的结核分枝杆菌特异性T淋巴细胞进行检测.结果 γ-干扰素释放分析T-SPOT.TB检测在结核性疾病阳性检出率为83.3%(20/24),明显高于结核菌素试验(tuberculin skin test,TST) 的41.7%(10/24)、涂片找抗酸杆菌的26.7%(4/15)、分枝杆菌分离培养的22.2%(2/9),差异有统计学意义(P＜0.05).γ-干扰素释放分析T-SPOT.TB检测诊断结核性疾病敏感性、特异性分别为83.3%、96.4%,显著优于结核菌素试验.结论 γ-干扰素释放分析T-SPOT.TB检测是诊断结核的快速敏感方法,在结核性疾病诊断中有重要价值.%Objective To evaluate the diagnostic value of an interferon-gamma release assay T-SPOT. TB in tuberculosis. Methods T-SPOT. TB assay reagent was employed to detect Mycobacterium tuberculosis antigen specific T cells in the peripheral blood samples from the patients with suspected tuberculosis. Results The positive detection rate was 83. 3%( 20/24 ) by interferon-gamma release assay T-SPOT. TB was significantly higher than 41. 7%( 10/24 ) by tuberculin test, 26. 1% (4/15 ) in smear for acid-fast bacillus and 22. 2% ( 2/9 ) in mycobacteria separation culture with statistical differences( P <0. 05 ). The sensitivity and specificity of interferon-gamma release assay T-SPOT. TB were 83. 3% and 96. 4% , which were significantly higher than those of tuberculin test with statistical ditfer-ences( P <0. 05 ). Conclusions Interferon-gamma release assay T-SPOT. TB is rapid and high-sensitive test for diagnosing tuberculosis.