靶向大鼠Caveolin-1基因的短发夹RNA表达载体的构建

摘要

Objective To construct short hairpin RNA expression vector targeting for rat caveolin-1. Methods Chemosyntheticoligos were annealed and then cloned into the pLL3. 7 plasmid to generate the recombinant plasmid pLL3. 7-cav-l which was confirmed byrestriction endonuclease digestion and DNA sequencing. Result The vector of pLL-3. 7-cav-l was constructed successfully. ConclusionThe shor hairpin RNA expression vector targeting for rat caveolin-1 has been constructed successfully, which facilitated to the study onrelationship between caveolin-1 and ALI/ARDS.%目的 构建靶向大鼠caveolin-1基因的shRNA表达载体.方法 化学合成靶向caveolin-1的单核苷酸链,经退火成双链.将退火得到的双链DNA克隆至表达载体pLL3.7中得到重组质粒,经限制性内切酶酶切、DNA测序进行鉴定.结果 通过限制性内切酶酶切、DNA测序证实,成功构建大鼠pLL3.7-cav-1表达载体.结论 成功构建了靶向大鼠caveolin-1的shRNA表达载体,为以后进行caveolin-1与ALI/ARDS的相关研究奠定了基础.