微管相关蛋白LC3-II参与川崎病炎症反应的研究

摘要

ObjectiveTo investigate the relationship between microtubule-associated protein l light chain 3B (LC3-II) and the inlfammatory response of Kawasaki disease (KD).MethodsThirty-nine cases of acute KD before intravenous admin-istration of immunoglobulin were enrolled. According to the results of echocardiography, the 39 cases were furtherly divided into KD with coronary artery lesion (CAL, 20 cases) group and KD with non-CAL (NCAL, 19 cases) group. At the same time, 12 healthy children were selected as controls. Serum samples were collected and cultured in vitro by human coronary artery endothe-lial cells (HCAEC) for 12 h. The LC3-II protein and mRNA expression of HCAEC were detected by Western-blotting and Q-PCR respectively.ResultsThe LC3-II protein and mRNA expression in CAL group and NCAL group were signiifcantly higher than those in control group, the LC3-II protein and mRNA expression in CAL group was higher than those in NCAL group, and both differences were statistically signiifcant (P<0.05).ConclusionsAutophagy may be involved in the inlfammatory response of KD in acute phase, which may be related to endothelial cell lesions of coronary artery in children with KD.%目的：探讨微管相关蛋白LC3-II与川崎病(KD)炎症反应的关系。方法选入39例急性期静脉注射丙种球蛋白前的KD患儿，根据心脏彩超结果，20例KD伴冠状动脉损伤(CAL)组，19例不伴冠状动脉损伤(NCAL)组；同时以12例正常儿童作为对照组。采集血清标本，加入人冠状动脉内皮细胞(HCAEC)体外培养体系干预12 h。采用Western-blotting、Q-PCR分别测定HCAEC中LC3-II蛋白及mRNA的表达。结果干预12 h后，CAL组、NCAL组的LC3-II蛋白及mRNA表达均较对照组明显升高，且CAL组更高于NCAL组，差异均有统计学意义(P<0.05)。结论自噬参与了急性期KD的炎症反应，可能与KD患儿冠状动脉内皮细胞损害有关。