Objective To observe the effects of interleukin (1L) -10 on expression of nuclear factor (NF) -kB , intercellular adhesion molecule( ICAM)-1 and neuronal apoptosis in brain tissue of rats with intraccrebral hemorrhage (1CH). Methods Ninty-six SD rats were randomly divided into the normal group, control group, IL-10 low-dose group and high-dose group, and the last 3 groups again randomly divided into 5 time points (6 h, 12 h,l d,3 d and 7 d). The iCH rat models were established by intracranial injection of Ⅷ collagenase. After 3 h of the model made, IL-10 5 μg/kg or 30 (μg/kg or normal saling was intraperitoneally injected to rats in IL-10 low-dose group, high-dose group and control group respectively. The expression of NF-kB and ICAM-1 in brain tissue around haematoma were detected by immunohistochemistry. The quantity of neuronal apoplosis was measured with situ end labeling method. Results Compared with the normal group, the quantity of positive cells of NF-kB, ICAM-1 and apoptotic cells around intracerebral haemaloma in other 3 groups at all the time points were significantly higher (all P < 0. 01 ). Compared with control group, the expression of NF-kB was began to reduce at 12 h, the expression of ICAM-1 and quantity of apoptotic cells were began to reduce at 1 d, and those changes at 7 d were the most obvious ( P < 0. 05 -0.01). Those, changes in IL-10 high-dose group were started from 12 h and the degree of the changes were more apparent (P < 0. 05 -0.01). Conclusions IL-10 can inhibit NF-kB and ICAM-1 expression in brain tissue, reduce the. inflammatory reaction and neuronal apoptosis around haematoma, play protective role in ICH damage. The high-dose of IL-10 has better protection.%目的 探讨白介素(IL)-10对脑出血大鼠脑组织核因子(NF)-kB、细胞间黏附分子(ICAM)-1表达及神经细胞凋亡的影响.方法 96只SD大鼠随机分为正常组、对照组、IL-10低剂量组和高剂量组,后3组再随机分为6h、12h、1d、3d及7d5个时间点.应用Ⅶ型胶原酶注射法制备脑出血模型；制模成功后3hIL-10低剂量组和高剂量组分别给予IL-10 5 μg/kg或30 μg/kg、对照组给予生理盐水腹腔注射,以后每天1次.应用免疫组化法测定各组血肿周围脑组织NF-kB及ICAM-1表达,原位末端标记法检测脑组织细胞凋亡.结果 与正常组比较,其他各组各时间点脑组织NF-kB、ICAM-1表达明显升高,神经细胞凋亡数量明显增多(均P＜0.01).与对照组比较,IL-10低剂量组NF-KB表达制模后12 h开始降低,ICAM-1表达及神经细胞凋亡数量自1d开始降低,7d时降低最显著(P＜0.05 ～0.01)；IL-10高剂量组上述变化在制模后12h开始,且下降程度更显著(P＜0.05～0.01).结论 IL-10可抑制脑组织NF-kB及ICAM-1表达,减轻血肿周边炎症反应及神经细胞凋亡,对出血性脑损伤起保护作用；高剂量组作用更明显.
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