结直肠癌术后化疗药物5-氟尿嘧啶、奥沙利铂和亚叶酸钙的测定

摘要

目的 结直肠癌患者尿液中5-氟尿嘧啶、奥沙利铂和亚叶酸钙3种常用药物含量的测定.方法 对4名结直肠癌患者尿样中5-氟尿嘧啶、奥沙利铂和亚叶酸钙3种药物残留量采用高效液相色谱法进行分离和分析,流速1 mL/min,流动相,pH=3磷酸水溶液/甲醇(80/20 v/v),检测波长270 nm,色谱柱Inertsil ODS(150 mm× 4.6 mm,5μm).结果 5-氟尿嘧啶、奥沙利铂和亚叶酸钙的检出限分别为42.0、29.4、33.3 μg/L,线性范围1.0～1 000.0 mg/L,相对标准偏差(RSD)分别为0.34％、0.98％、1.00％,回收率93.40％ ～～110.70％.结论 该方法样品处理简便,色谱分离完全,结果准确可靠,为结直肠癌患者尿液中的药物代谢组学研究提供了基础.%Objective To investigate the detection of the amount of 5-fluorouracil,leucovorin calcium and oxaliplatin in the urine of colorectal cancer patients.Methods The urine of 4 colorectal cancer patients was used by HPLC to separate and analyse the three above mentioned drugs with flow rate (1 mL/min),mobile phase (phosphoric acid solution/methanol (80/20 v/v)),detection wavelength (270 nm) and column (Inertsil ODS (150 mm × 4.6 mm,5μm)).Results The detection limits of 5-fluorouracil,leucovorin calcium and oxaliplatin were 42.0,29.4,33.3 μg/L,respectively.The linear range was 1.0-1 000.0 mg/L.The relative standard deviation was 0.34 ％,0.98 ％,1.00 ％,and recovery rate was between 93.40 and 110.70 ％.Conclusion This method is simple,inertsil being fully separated,the result being reliable,which provides a base for future metabolomics research in the urine of patients with colorectal cancer.