接骨七厘片、辛伐他汀对促进骨折愈合的动物实验研究

摘要

目的 探讨接骨七厘片和辛伐他汀对促进骨折愈合的机制,并分析联合应用接骨七厘片和辛伐他汀与单独应用接骨七厘片或辛伐他汀对促进骨折愈合的差异.方法 用蒸馏水将接骨七厘片和辛伐他汀片分别溶解,配成一定浓度的溶解液.将实验大鼠随机分成4组,将双侧尺桡骨中1/3段徒手折断,造成闭合性完全性骨折,不给予外固定.术后接骨七厘片组给予接骨七厘片溶液,1次/d,连续28 d；辛伐他汀片组给予辛伐他汀溶液,1次/d,连续28d;联合应用组同时给予接骨七厘片溶液及辛伐他汀溶液,1次/d,连续28d；对照组灌胃给予生理盐水1 mL,1次/d,连续28d.结果 在一般行为表现、X线表现、组织形态学、骨痂干重等方面,接骨七厘片组和(或)辛伐他汀组与联合应用接骨七厘片和辛伐他汀组相比,差异有统计学意义(P＜0.05).结论 本实验研究发现接骨七厘片及辛伐他汀在骨折愈合的过程中,均能增加成骨细胞的活性,使成骨细胞大量增生,促进骨痂的形成,提高骨痂的质量,而联合应用接骨七厘片及辛伐他汀比单纯使用接骨七厘片或辛伐他汀片作用更明显,骨折愈合较快.%Objective To analyze the mechanism of Jieguqili chip and simvastatin in promoting fracture healing, and to compare the difference between the combined use of the Jieguqili chip and simvastatin and single use of Jieguqili chip or simvastatin in promoting fracture healing. Methods The Jieguqili chip and simvastatin tablets were dissolved with distilled, dubbed a certain concentration of solvent. Rats were randomly divided into 4 groups, and their bilateral radius and ulna 1/3 of unarmed were broken, which created the closed complete bone fracture. No external fixation was done. After the technique, the 1st group was treated the Jieguqili chip solution, once a day, continually 28 days. The second group was treated with simvastatin solution, once a day, continually 28 days. The third group was treated with Jieguqili chip solution combined with simvastatin solution, once a day, continually 28 days. The 4th group was the control group, whose stomachs were filled physiological saline 1 mL, once a day, continually 28 days. Results In terms of the general performance, X performance, histomorphology, callus dry weight, the Jieguqili chip group and simvastatin group showed statistical difference with the combined group (P<0. 05). Conclusion This study suggests that the Jieguqili chip and simvastatin in the process of fracture healing, can increase the osteoblast activeness, cause the osteoblast massive proliferations, promote of bone formation of scabs, and improve the quality of callus. The combined use of Jieguqili chip and simvastatin is better in promoting fracture healing than the single use of Jieguqili chip or simvastatin.