类风湿关节炎患者关节液中miRNA表达谱研究及其临床意义

摘要

Objective To detect the gene expression profiles of miRNA in joint fluids of patients with rheumatoid arthritis(RA)and healthy people and clinical significance.Methods The expressions of miRNAs in joint fluids of 3 RA patients and 3 healthy adults were detected by miRNA chip technology,and miRNAs with significant differential expressions were selected to be validated by the qRT-PCR.Correlations between selected miRNA and clinical and laboratory indexes of RA were analyzed.Results In the gene expression profile of miRNA in joint fluids of patients with RA,the expressions of 23 miRNAs significantly changed when compared with those in the healthy people.The results of selected miRNAs by qRT-PCR were correspond with the Results of the miRNA chip technology.The difference of miR-146a expression in joint fluid between RA patients and healthy controls was the most significant.Pearson correlation analysis showed that serum miR-146a expression in joint fluid of RA patients was positively correlated with rheumatoid factor(RF)value and DAS28 score.ROC curve analysis showed that the area under the curve(AUC)of miR-146a was 0.71,and the sensitivity and the specificity of miR-146a in joint fluid in predicting RA were 85.29％and 61.54％respectively.Conclusion There is significant difference in expression of miRNAs in joint fluid between RA patients and healthy people.Expression of miR-146a increases significantly in joint fluid of RA patients,and it level is significantly correlated with the clinical and laboratory indexes of RA.%目的 检测类风湿关节炎(RA)患者与健康对照者关节液中miRNA表达谱差异及临床意义.方法 应用miRNA芯片技术检测3例RA患者和3例正常人关节液中miRNAs表达情况,筛选出表达差异显著的miRNAs,并采用实时荧光定量PCR(qRT-PCR)验证.进一步研究候选miRNA与RA临床和实验室指标的相关性.结果 RA患者关节液miRNA表达谱中23个miRNA较健康对照者发生显著改变.qRT-PCR检测结果与芯片结果相符.RA患者与健康对照者关节液中miR-146a的表达差异最为显著.Pearson相关分析显示,RA患者关节液中miR-146a的表达与类风湿因子(RF)和DAS28评分呈正相关.ROC曲线分析结果显示,miR-146a曲线下面积(AUC)为0.71,关节液miR-146a预测RA的灵敏度为85.29％,特异度为61.54％.结论 RA患者关节液与健康人关节液存在表达差异显著的miRNAs.miR-146a在RA患者关节液中表达显著升高,其水平与RA的临床和实验室指标存在显著相关性.