Objective To investigate the changes in peripheral blood dendritic cell (DC) subsets in patients with different stages of hepatitis B virus (HBV)-associated disease and related clinical significance.Methods A total of 20 HBV carriers (HBV carrier group),20 chronic hepatitis B (CHB) patients (CHB group),20 hepatitis B cirrhosis patients (cirrhosis group),and 20 primary liver cancer patients (liver cancer group),all of whom had HBV-associated disease and were selected from Department of Hepatology,Longhua Hospital Affiliated to Shanghai University of Traditiond Chinese Medicine,as well as 20 healthy volunteers (normal group),were included in this study flow cytometry was used to measure the changes in DC subsets.Comparison between multiple groups using Druskal-Wallis H test and further comparison using of Wilcoxon Rant test.Results According to the results of myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs) in patients with different types of HBV infection and normal population,there was a significant difference in the number of mDC between patients with different typers of HBV infection and normal population(x2 =33.629,P ＜0.001),and the cirrhosis group and the liver cancer group had significantly lower numbers of mDCs in peripheral blood than the normal group,CHB group,and HBV carrier group (all P ＜ 0.05).There was a significant difference in the number of pDC between patients with different types of HBV infection and normal population(x2 =13.602,P ＜ 0.009),and the liver cancer group had a significantly lower number of pDCs in peripheral blood than the normal group,CHB group,and HBV carrier group (all P ＜ 0.05).The cirrhosis group had a significantly lower number of pDCs in peripheral blood than the CHB group (P ＜0.05).Conclusion There are significant differences in the expression of DC subsets between patients with different HBV-associated diseases,and the number of DCs may be used as a reference for evaluating disease progression and prognosis.%目的 探讨HBV相关疾病患者外周血树突状细胞亚群在不同病程中的变化及临床意义.方法 选取上海中医药大学附属龙华医院肝病科HBV携带者(HBV携带组)、CHB患者(CHB组)、乙型肝炎肝硬化患者(肝硬化组)、原发性肝癌患者(肝癌组)(均为HBV相关疾病)及健康志愿者(正常组)各20例,采用流式细胞分析其外周血树突状细胞(DC)亚群的变化.多组间比较采用kruskal-wallisH检验,进一步两两比较采用Wilcoxon秩和检验.结果 不同类型HBV感染者与正常人外周血髓样树突细胞(mDC)和浆细胞样树突细胞(pDC)比较结果显示,不同类型的HBV感染者与正常组之间mDC数量比较差异有统计学意义(x2=33.629,P＜0.001),进一步比较发现肝硬化组、肝癌组患者外周血mDC的数量均低于正常组、CHB组和HBV携带组,差异均有统计学意义(P值均＜0.05);不同类型的HBV感染者与正常组之间pDC数量比较差异有统计学意义(x2=13.602,P=0.009),进一步比较发现肝癌组患者外周血pDC数量均低于正常组、CHB组和HBV携带组,差异有统计学意义(P值均＜0.05);肝硬化组患者外周血pDC数量低于CHB组,差异均有统计学意义(P＜0.05).结论 慢性HBV感染不同阶段的患者其DC亚群表达存在显著差异,其中肝硬化和肝癌患者DC亚群数量最少,DC的数量可能是病情演变和预后评判的参考依据.
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