无刚毛鳞甲复合体同源物样蛋白2在慢性丙型肝炎患者中的表达及其与血清球蛋白的关系

摘要

Objective To investigate the correlation between peripheral CD4 +CXCR5 +PD -1 +T cells and serum globulin in chronic hep-atitis C (CHC)patients and health volunteers,and to clarify the role of the expression of achaete -scute homologue 2 (Ascl2),the tran-scription factor in peripheral follicular helper T (Tfh)cells,in the process of chronic hepatitis C virus (HCV)infection.Methods A total of 46 previously untreated CHC patients who were admitted to Tangdu Hospital,Fourth Military Medical University,from October 2015 to May 2016 were enrolled,and 32 healthy laboratory technicians and persons who underwent physical examination in our hospital were enrolled as healthy control group.Flow cytometry was used to measure the percentage of Tfh cells in CD4 + T cells and the change in its subset (CD4 +CXCR5 +PD -1 + T cells)in peripheral blood,an automatic biochemical analyzer was used to measure the serum globulin level, and real -time PCR was used to measure the mRNA expression of Ascl2 in Tfh cells.The association between the changes in these parame-ters and HCV infection was analyzed.The independent samples t -test was used for the comparison of continuous data between groups,and the Pearson correlation analysis was also performed.Results In CHC patients,peripheral Tfh cells were positively correlated with B lym-phocytes (r =0.582 3,P =0.011 2),peripheral B lymphocytes were positively correlated with globulin (r =0.450 9,P =0.031 6),and Tfh cells were positively correlated with globulin (r =0.583 5,P =0.038 3).CHC patients had significantly higher mRNA expression of Ascl2 in peripheral Tfh cells than the healthy control group (1.019 ±0.666 vs 6.437 ±5.776,t =4.552,P =0.001 9).Conclusion Tfh cells may be involved in the production of serum globulin in CHC patients,and the transcription factor Ascl2 may participate in the differenti-ation and development of Tfh cells in the process of HCV infection.%目的：探讨慢性丙型肝炎（CHC）患者和健康志愿者外周血 CD4＋CXCR5＋PD －1＋T 淋巴细胞与血清球蛋白之间的相关性，明确外周血滤泡辅助性 T 淋巴细胞（Tfh）转录因子无刚毛鳞甲复合体同源物样蛋白（Ascl）2的表达在慢性 HCV 感染过程中的作用。方法选取2015年10月－2016年5月第四军医大学唐都医院收治的住院或门诊初治 CHC 患者46例，另选取实验室健康成年实验人员以及本院健康体检者32例作为健康对照组。应用流式细胞技术分析外周血中 Tfh 细胞在 CD4＋T 淋巴细胞中所占比例及其亚群（CD4＋CXCR5＋PD －1＋T 淋巴细胞）变化，应用全自动生化分析仪检测血清球蛋白水平，并通过 real －time PCR 检测Tfh 细胞中 Ascl2 mRNA 表达情况；分析各指标变化与 HCV 感染的相关性。计量资料组间比较采用独立样本 t 检验，相关性分析采用 Pearson 相关分析。结果 CHC 患者外周血 Tfh 细胞与 B 淋巴细胞呈正相关（r ＝0．5823，P ＝0．0112），外周血 B 淋巴细胞与球蛋白呈正相关（r ＝0．4509，P ＝0．0316），Tfh 细胞与球蛋白呈正相关（r ＝0．5835，P ＝0．0383）；CHC 患者外周血 Tfh 细胞中 Ascl2 mRNA 表达显著高于健康对照组（1．019±0．666 vs 6．437±5．776，t ＝4．552，P ＝0．0019）。结论 Tfh 细胞与 CHC 患者血清中球蛋白的产生有一定的联系，转录因子 Ascl2可能参与 HCV 感染过程中 Tfh 细胞的分化发育。