乳腺癌中 THRβ1表达与甲状腺激素改变的临床研究

摘要

Objective To explore patients with breast cancer of serum thyroid hormone level changes,and to investigate thyroid hormone receptorβ1(THRβ1)in breast cancer tissues and its relationship with clinicopathological factors relate to one another. Methods ①51 breast cancer patients were selected for the observation and were considered as breast cancer group. 51 normal patients were considered as the control group. Their serum thyroid hormone(T3 ,T4 ,TSH)was examined and then carry out comparative analysis. ②RT - PCR was used to detect the expression of THRβ1mRNA in 51 human breast cancer and 51 normal breast tissues. Results ①The content of T3 in serum of the breast cancer patients is decreased compared with that of normal group. ②A positive rate of THRβ1mRNA was 37. 2% in human breast cancer and 87. 2% in normal breast tissues. The expression level was lower in breast cancer than that of normal breast tissues( P ﹤ 0. 05). The expression was not cor-related with patients' age,histologic types. The expression level was lower in late - stage tumors(stage III)than that of early - stage tumors(stage I and II),in tumors with lymph node metastasis than those without lymph node metastasis,and in large - sized tumors than that of small - sized ones. Conclusion The content of T3 serum of the breast cancer patients is decreased. THRβ1 might play all important role in the development of invasion and metastasis of breast cancer.%目的：探讨乳腺癌患者血清中甲状腺激素水平的变化，研究甲状腺激素受体 THRβ1在乳腺癌组织中的表达及其与临床病理因素之间的相互关系。方法①51例乳腺癌患者为观察组，对照组为51例健康女性，统计其血清中甲状腺激素（T3、T4、TSH）水平，并结合患者临床病理指标进行分析。②选51例乳腺癌组织和51例正常组织，应用RT - PCR 琼脂糖凝胶电泳检测标本中 THRβ1mRNA 表达情况，分析与临床病理的关系。结果①乳腺癌患者血清中甲状腺激素 T3水平下降。肿瘤直径越大、有淋巴结转移、临床分期越晚，其 T3降低越显著。②在51例乳腺癌组织中， THRβ1 mRNA 19例表达阳性，阳性率为37.2%；而在51例癌旁正常乳腺组织中45例表达阳性，阳性率为88.2%。在腺癌组中表达率明显低于正常乳腺组（ P ﹤0.05）。THRβ1 mRNA 低表达与年龄、病理类型无关（ P ﹥0.05），而与淋巴结转移、肿瘤大小、TNM 分期显著相关（ P ﹤0.05）。结论乳腺癌患者血清甲状腺激素 T3水平下降，T4、TSH 无明显变化。且肿瘤直径越大、有淋巴结转移、临床分期越晚，其 T3降低越显著；THRβ1mRNA 的低表达在乳腺癌的发生中起一定作用。提示乳腺癌与甲状腺激素之间存在着相关性。