microRNA 在肝癌组织中的表达及其潜在临床价值分析

摘要

Objective To study the expression of microRNA in hepatocellular carcinoma tissue and analyze its potential clinical value. Methods Data of 120 cases of liver cancer were collected and classified into stageⅠ,Ⅱ,Ⅲ and Ⅳ according to the TNM classification. The to-tal RNA was extracted from cancer and adjacent normal tissue and the first strand cDNA was obtained by reverse transcription after miRNA modifi-cation by poly A tailing. The content of miRNA was detected by fluorescence quantitative PCR. Results The contents of miR - 122,miR - 199 and miR - 451 in hepatocellular carcinoma were less than normal tissue,and negatively correlated with TNM stage;the contents of miR - 181 and miR - 21 were higher than normal tissue,and positively correlated with TNM. Conclusion miRNA levels are correlated with TNM stage of hepa-tocellular carcinoma and can be used as an examination index in early diagnosis of hepatocellular carcinoma.%目的：研究微小 RNA（microRNA，miRNA）在肝癌组织中的表达及其潜在临床价值。方法收集120例肝癌患者资料，按照 TNM 分期分为Ⅰ期、Ⅱ期、Ⅲ期和Ⅳ期。采用 miRNA 提取分离试剂盒提取肝癌组织和癌旁正常组织中的总 RNA 并通过 polyA 尾修饰法反转录 miRNA 为 cDNA 第一链，进而通过荧光定量 PCR 的方法检测 miRNA 的含量。结果肝癌组织中 miR -122、miR -199、miR -451含量低于正常组织，且与 TNM 分期呈负相关；miR -181、miR -21含量高于正常组织，且与 TNM 分期呈正相关。结论 miRNA 含量与肝癌 TNM 分期相关，可作为早期诊断肝癌的辅助检查指标。