原发性肝癌组织MicroRNA-497表达及与患者临床特征和生存率的关系

摘要

Objective:To explore the value of the microRNA-497 expression and clinical significance in hepa-tocellular carcinoma(HCC) .Method:Paired HCC tumor tissues and adjacent non-tumor liver tissues were collected from 61 HCC patients who underwent hepatectomy between January 2010 and October 2010 at our hospital .miR-NA-497 was quantified using the RT-PCR .61 patients were divided into miRNA-497 high expression group (n=21) and miRNA-497 low expression group (n=40) ,according to the limit of the median expression level of miRNA-497 in tumor tissues .Then ,we compared the difference of miRNA-497 levels between cancer tissues and adjacent tis-sues and analyzed the difference of clinical data and survival rate between miRNA-497 high expression group and miRNA-497 low expression group and found the risk factors of prognosis of patients with HCC .Results:The level of miR-497 was significantly down-regulation compared to paracarcinomatous liver tissues (P< 0 .05) ,miR-497 ex-pression was also negatively correlated with serum AFP level ,tumor size ,TNM stage and venous invasion (all P<0 .05) .In addition ,Kaplan Meier survival analysis showed that the 1-,3-,and 5-year overall survival rates were lower in the low miR-497 exprssion than the high miR-497 exprssion ( 55 .0% ,20 .0% ,15 .0% vs 71 .4% ,42 . 9% ,37 .5% ,P<0 .05) .Multivariable Cox regression analysis revealed that miR-497 ,TNM stage and venous invasion were independent risk factors for overall survival .Conclusion:miR-497 was down-regulated in HCC and can be served as a novel prognostic marker for HCC .%目的:回顾性分析microRNA-497(miRNA-497)在原发性肝癌(HCC)患者癌组织中的表达及与患者临床特征和生存率的关系.方法:收集2010-01—2010-10接受手术治疗的61例HCC患者,采用荧光定量PCR检测其癌组织及癌旁组织miRNA-497的相对表达量.以癌组织miRNA-497相对表达水平的中位数为界限,将61例患者分为miRNA-497高表达组(n=21)和miRNA-497低表达组(n=40).比较癌组织与癌旁组织miRNA-497水平差异;同时分析miRNA-497高表达组与miRNA-497低表达组临床特征和预后危险因素;比较两组平均生存率和累计生存率的差异.结果:miRNA-497在癌组织中的表达明显低于癌旁组织,差异有统计学意义(P<0.05).HCC患者癌组织miRNA-497低表达组血清AFP水平、肿瘤大小、TNM分期、血管侵犯与高表达组分布差异有统计学意义(P<0.05).多因素回归分析显示血管侵犯、TNM分期、miRNA-497表达量是影响HCC患者预后的独立危险因素.Kaplan-Meier分析表明,miRNA-497低表达组平均生存期明显低于高表达组(19.0个月vs 33.3个月),1年、3年、5年累计生存率也较miRNA-497高表达组明显降低(55.0%vs 71.4%、20.0%vs 42.9%、15.0%vs 37.5%,P<0.05).结论:miRNA-497在HCC组织中的低表达提示预后不良.