miR-221/222基因、ERα在阴道前壁膨出患者阴道前壁中的表达

摘要

目的 探讨miR221和miR222基因、雌激素受体 α(ERα)在阴道前壁膨出(AVP)患者阴道前壁组织中的表达.方法 选择AVP进行手术治疗的40例患者为AVP组,同期因其他妇科疾病进行手术的40例患者作为对照组,两组包含绝经前组与绝经后组各20例.术中取患者阴道前壁尿道中段处组织,分别用RT-PCR、Western blot方法检测两组miR-221/222、ERα的表达.结果 AVP组患者绝经前、绝经后miR-221和miR-222的表达较对照组显著升高(P<0.05),且ERα的表达明显低于对照组(P<0.05).结论 miR221和miR222可能是盆腔脏器脱垂的致病基因,它们的高表达抑制了ERα,参与AVP发生及进展.%Objective To explore the clinical significance and relativity of miRNA-221 / 222 and estrogen receptor α in the patients with anterior vaginal prolapse (AVP). Methods 40 cases with anterior vaginal prolapsed who underwent surgery were analyzed. In the same period, 40 cases with other gynecological diseases who underwent surgery served as the control group. Each group contains 20 premenopausal and 20 post-menopausal. The mid-urethral tissues of the anterior vaginal wall of all cases were acquired in surgery. Expression of miRNA-221 / 222 was de-termined by RT-PRT,and ERα expression was tested by Western blot. Results MiRNA-221 / 222 expression was significantly increased in women of premenopausal and postmenopausal with AVP compared to the control group (P < 0. 05);ERα in AVP patients was significantly lower than that in the control group (P < 0. 05). Conclusion MiR221 / miR222 probably is causative genes of pelvic organ prolapse (POP). The ele-vation of their expression can inhibit ERα,and participate in the development of AVP.