微小染色体维持蛋白在膀胱肿瘤组织和尿脱落细胞中的表达及对膀胱肿瘤复发检测预后价值

摘要

目的 探讨微小染色体维持蛋白(MCM5)在膀胱肿瘤组织和尿脱落细胞中的表达及对膀胱肿瘤复发检测预后价值.方法 选取2016～2017年膀胱癌患者72例、泌尿系统疾病者60例和健康体检者60例资料进行回顾性分析,均取膀胱组织标本和尿脱落细胞标本进行MCM5检测.并进行尿细胞学检查,比较不同检查方法的特异度、敏感度.结果 在膀胱癌患者膀胱肿瘤组织中,随着病理分级、临床分期增加,其MCM5表达量升高,差异有统计学意义(P<0.05);在膀胱癌尿脱落细胞中,有64例(88.89％)MCM5表达阳性,与对照组和泌尿系统疾病组比较,膀胱癌患者尿脱落细胞中MCM5表达水平升高,差异有统计学意义(P<0.05);随着病理分级及临床分期增加,膀胱癌患者尿脱落细胞中MCM5表达量升高,差异有统计学意义(P<0.05);但在MCM5阳性表达方面差异无统计学意义(P<0.05);且初发和复发患者MCM5阳性率比较差异无统计学意义(P>0.05);尿细胞学检查与MCM5检测的特异度比较差异无统计学意义(P>0.05),与尿细胞学检查比较,MCM5检测的敏感度升高,差异有统计学意义(P<0.05).结论 随着病理分级及临床分期增加,膀胱癌患者膀胱肿瘤组织和尿脱落细胞中MCM5表达量升高,膀胱癌患者进行MCM5检测,对检测膀胱肿瘤组织复发及预后判断有重要作用.%Objective To investigate the expression of micro-chromosome maintenance protein (MCM5)in bladder tumor tissues and u-rinary exfoliated cells and its prognostic value of bladder tumor recurrence. Methods 72 cases of bladder cancer,60 cases of urinary system dis-eases and 60 cases of healthy physical examination in our hospital from January 2016 to 2017 were selected and taken their samples of bladder tis-sue and urine exfoliated cells for MCM5 examination and cytological examination. The specificity and sensitivity of different examinations were com-pared. Results For patients with bladder cancer,the expression of MCM5 increased with the increase of pathological grading and clinical staging, and the difference was statistically significant (P < 0. 05). In the aspect of bladder cancer urinary exfoliated cells,64 cases (88. 89％)had posi-tive expression of MCM5,and compared with the control group and the urinary system disease group,the expression level of MCM5 in urinary exfo-liated cells of bladder cancer patients was increased,the difference was statistically significant (P < 0. 05). With the increase of pathological grading and clinical staging,the expression of MCM5 in urinary exfoliated cells of bladder cancer patients was increased,the difference was statisti-cally significant (P < 0. 05),but there was no significant difference in MCM5 positive expression (P < 0. 05). And there was no significant difference in the positive rate of MCM5 between primary and recurrent patients (P > 0. 05). The comparison between the specificity of the urine cytology test and the MCM5 test was not statistically significant (P > 0. 05). Compared with the urine cytology test,the sensitivity of MCM5 de-tection was increased,the difference was statistically significant (P < 0. 05). Conclusion With the increase of pathological grade and clinical stage,the expression of MCM5 in bladder cancer tissue and urinary exfoliated cells increase in bladder cancer patients. MCM5 was detected in bladder cancer patients,which had an important effect on the detection of bladder tumor recurrence and prognosis.