钙拮抗剂对大鼠脑缺血后血-脑脊液屏障通透性的影响

摘要

目的：观察钙拮抗剂对大鼠脑缺血后血-脑脊液屏障（ BBB）通透性的影响。方法制备脑缺血再灌注（ CIR）大鼠模型150只，在大鼠缺血2 h后进行再灌注。将大鼠平均分为观察组（造模后腹腔注射尼莫地平）与对照组（造模后腹腔注射生理盐水），在6 h、12 h、24 h、48 h、72 h时间点分别进行再灌注，观察每个时间点BBB通透性损伤情况与梗死灶体积百分比。结果随着时间的延长，CIR后BBB通透性与梗死灶体积百分比出现逐步上升，且在12 h、48 h达到双高峰。观察组的BBB通透性及脑梗死灶体积百分比的上升较对照组较为明显（ P ﹤0.05）。结论大鼠实验结果提示，CIR能够提升BBB的通透性，增加梗死灶体积百分比，而早期使用钙拮抗剂能够加重上述情况。%Objective To observe the effect of calcium antagonist on the permeability of blood-cerebral barrier in rats with cerebral is-chemia. Methods 150 rats models of cerebral ischemia reperfusion( CIR)were prepared and reperfused after 2 h of ischemia. These rats were divided into observation group( intraperitoneal injection of nimodipine after modeling)and control group( intraperitoneal injection of normal saline after modeling),reperfusion was performed at 6 h,12 h,24 h,48 h and 72 h time points respectively. The damage of permeability of blood-cer-ebral barrier and percentage of infarct volume had been observed at each time point. Results With the prolongation of time,the percentage of permeability of blood-cerebral barrier and volume of infarction had been gradually increased after CIR,and reached the peak at 12 h and 48 h. The permeability of blood-cerebral barrier and the percentage of volume of infarction in rats of observation group were significantly higher than those of rats in control group( P ﹤0. 05). Conclusion The above results suggest that CIR can enhance the permeability of blood-cerebral bar-rier,increase the percentage of infarction size,and the early application of calcium antagonists can aggravate such situation.