大鼠急性肝缺血-再灌注时心肌细胞氧化损伤及瑞芬太尼的干预作用

摘要

目的 观察大鼠急性肝缺血-再灌注时心肌细胞氧化损伤以及瑞芬太尼预处理对氧化损伤的干预作用.方法 建立大鼠肝缺血-再灌注损伤模型.将72只Wistar大鼠随机分为瑞芬太尼预处理组(R组,n=24)、缺血-再灌注组(IR组,n=24)、假手术组(Sham组,n=24).于再灌注30min、1、2、3 h处死大鼠.R组缺血前以1μg·kg-1·min-1微泵输注瑞芬太尼30 min进行预处理.分别检测各组大鼠心肌组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力及谷胱甘肽过氧化氧酶(GSH-Px)活力变化.结果 与Sham组比较,R组和IR组再灌注1、2、3 h时心肌组织MDA含量明显升高,SOD、GSH-Px活力明显降低(P<0.05).与IR组比较,R组再灌注30 min、1 h时心肌组织MDA含量降低,SOD、GSH-Px的表达增高(P<0.05).结论 大鼠急性肝缺血-再灌注可造成心肌细胞氧化损伤,而瑞芬太尼预处理可起到一定的保护作用.%Objective To observe the oxidative damage of myocardial cells in rats with acute hepatic ischemia-reperfusion injury and the intervention of remifentanil pretreatment. Methods Establish acute hepatic ischemia-reperfusion injury model. Seventy-two wistar rats were randomly divided into sham operation group (Sham group), ischemia-reperfusion group (IR group ),remifentanil pretreatment group (R group). Then rats were killed respectively in 30 min, 1, 2, 3 h of reperfusion. R group rats with intravenous infusion for 30 min,at 30 min before ischemia-reperfusion at 1 μg·kg-1 ·min-1 ). The other steps were the same as IR group. Then, detect the MDA, SOD,GSH-Px content of the myocardial tissue in all groups. Results Compared with the Sham group, the MDA content of myocardial tissue in IR group and R group increased, the SOD, GSH-Px content decreased(P＜0.05) in 1,2,3 h. Compared with IR group, the MDA content of myocardial tissue in R group decreased significantly(P＜0.05 )and the SOD, GSH-Px content increased significantly( P＜ 0.05). Conclusion Oxidative damage of myocardial cells was observed in rats with acute hepatic ischemia-reperfusion injury. Remifentanil pretreatment play a protective role in the forenamed process.