头穴丛刺对慢性脑缺血大鼠认知功能障碍及VEGF蛋白表达的影响

摘要

Objective:To observe the effect of cluster needling of scalp point on cognitive function and expression of VEGF in rats with chronic cerebral ischemia,and to explore the mechanism. Methods:To make the chronic ischemia model through improved permanent bilateral carotid arteries occlusion. The surviving rats were randomly divided into three groups :the group of ischemia,the group of cluster needling,the group of Nimodipine. To check the ability of leaning and remembering through Morris water labyrinth; to study the morphologic changes with HE staining and the expression of VEGF by immnunohistochemisty method. Results:The escape latency in each trial of the model group were longer than the sham operation ( P < 0. 05 ). The expression of VEGF in the cortex of frontal lobe and the hippocampus of rats of the group of cluster Needling and the group of Nimodipine were more than those in the group of ischemia (P < 0. 05). Conclusion: The cluster needling of scalp point can reduce the cases of ischemia and anoxia and perfect the state of cognitive impairment through up - regulation the expression of VEGF in rats with chronic cerebral ischemia.%目的:观察头穴丛刺对慢性脑缺血大鼠认知功能障碍及VEGF表达的影响,并探讨其作用机制.方法:采取国内改进的双侧颈总动脉永久性结扎法制备慢性脑缺血大鼠模型,制备成功的大鼠随机分为3组:缺血组、头穴丛刺组、尼莫地平组.Morris水迷宫检测大鼠学习记忆能力,HE染色观察大鼠额叶皮层和海马区的细胞形态变化,免疫组化检查VEGF蛋白表达的情况.结果:与假手术组相比,缺血组大鼠逃避潜伏期明显延长(P＜0.05)；与缺血组大鼠比较,头穴丛刺组和尼莫地平组大鼠额叶皮层和海马区VEGF蛋白表达明显增多(P＜0.05).结论:头穴丛刺法能上调VEGF在慢性脑缺血大鼠中的表达,减轻大鼠的缺血缺氧情况,改善慢性脑缺血大鼠认知功能障碍的状况.