电针联合脂肪源性干细胞移植对脑缺血/再灌注大鼠IGF-1及其受体表达的影响

摘要

Objective: To investigate the effect of electroacupuncture ( EA) combined with adipose - derived stem cell ( ADSC) transplanted on IGF - 1 and IGF - 1R in hippocampus after cerebral ischemia - reperfusion in rats. Methods: Adult SD rats were randomly divided into normal group, model group, EA group, ADSC group and EA plus ADSC group. Neurological function score was observed by NSS. The expressions of IGF - 1 and IGF - 1R in hippocampus were observed by using Western bolt and quantitative real - time RT - PCR. Results; The number of PKH -26 labeled ADSC in EA plus ADSC group was higher than that in ADSC group. Compared with EA group and ADSC group, the protein and mRNA of IGF - 1 and IGF - 1R were increased in EA plus ADSC group. However, the NSS was significantly decreased. Conclusion: The combined treatment of electroacupuncture plus ADSC promotes functional outcome significantly and the efficacy is better than either of the single treatment, which may be related to EA upregulating IGF - 1 and IGF -1R expressions in hippocampus, and promoting ADSC survival and migration.%目的:探讨电针联合脂肪源性干细胞(ADSC)移植对大鼠缺血/再灌注损伤后海马区胰岛素样生长因子1(IGF-1)及其受体(IGF-1R)表达的影响.方法:成年SD大鼠随机分为正常组、模型组、电针组、ADSC组、电针加ADSC组.NSS法行神经功能损害评分,Western bolt法及实时荧光定量PCR分别检测海马区IGF-1、IGF-1R蛋白和mRNA表达.结果:电针加ADSC组海马区PKH-26标记的细胞个数高于ADSC组.电针加ADSC组较电针组、ADSC组NSS评分显著降低,而海马区IGF-1和IGF-1R蛋白和mRNA表达显著增加.结论:电针加ADSC联合治疗协同促进脑缺血大鼠神经功能恢复,其机制可能与电针上调海马区IGF-1和IGF-1R的表达、改善干细胞生存内环境、促进移植的ADSC迁移和存活有关.