LOX-1介导苯扎贝特对内皮细胞凋亡的影响

摘要

目的 研究苯扎贝特对氧化型低密度脂蛋白(ox-LDL)诱导人脐静脉内皮细胞(HUVECs)凋亡的影响及血凝素样氧化型低密度脂蛋白受体1(LOX-1)在其中的作用.方法 用流式细胞仪观察HUVECs凋亡,用RT-PCR技术观察LOX-1基因表达的变化.结果 ox-LDL组与正常组比较凋亡明显增加(P<0.05),LOX-1 mRNA表达明显增加(P<0.05),苯扎贝特干预后凋亡减少,LOX-1 mRNA的表达降低(P<0.05),呈浓度依赖关系.用LOX-1阻滞剂多聚肌苷酸预先干预2 h后与苯扎贝特组比较细胞凋亡及LOX-1mRNA表达进一步减少(P<0.05).结论 苯扎贝特可改善ox-LDL对内皮细胞的影响,下调LOX-1表达,减少内皮细胞凋亡,部分机制可能通过LOX-1介导.%Objective To observe the influence of bezafibrate on the apoptosis and expression of lectin-like oxidized low density lipoprotein receptor-1(LOX-1) mediated by oxidized low density lipoprotein(ox-LDL) in cultured human umbilical vein endothelial cells(HUVECs).Methodes The apoptosis of HUVECs mediated by ox-LDL were evaluated by flow cytometry and the expression of LOX-1 mRNA were detected by RT-PCR.Results Compared with the control group,ox-LDL could increase apoptosis and the expression of LOX-1(P<0.05),and Bezafibrate could decrease the apoptosis and the expression of LOX-1 in a concentration -dependent manner(P<0.05).Preincubation of HUVECs with polyinosinic acid for 2 hours,the apoptosis and the expression of LOX-1 decreased(P<0.05).Conclusions Bezafibrate inhibits the apoptosis of HUVECs mediated by ox-LDL by reducing the expression of LOX-1,which may be part of the reasons for bezafibrate to prevent and treat atherosclerosis.