Aim An HPLC method for analyzing eleutheroside B (ELU B) and eleutheroside E (ELU E), two of the main active substances of Acanthopanax preparations were studied. Methods The samples were analyzed on a kromasil ODS column with water-acetonitrile as a gradient mobile phase. The flow rate was 0.8 mL·min-1 and detecting wavelengths were 206 nm for ELU B, 220 nm for ELU E, solid phase extraction (SPE) and internal standard-salicin were selected. Results The recoveries of Acanthopanax tablets and injection were 90.4%-96.8% and 96.4%-99.8% for ELU B, 87.7%-93.3% and 95.7%-98.5% for ELU E, respectively. The linear ranges were 4.45-22.25 μg· mL-1(r=0.999 8) and 5.11-25.55 μg·mL-1 (r=0.999 7) respectively. Conclusion This method can save the time for cleaning the chromatographic system and improve sensitivity for Acanthopanax preparations, thus providing a way to evaluate the quality of Acanthopanax preparations.%目的用高效液相色谱法研究刺五加制剂中2个主要活性成分刺五加苷B、苷E的含量测定方法.方法 Kromasil ODS柱, 水-乙腈梯度流动相, 流速0.8 mL·min-1, 测定波长刺五加苷B 206 nm,刺五加苷E 220 nm, 水杨酸作为内标, 选择了固相萃取条件.结果刺五加片中刺五加苷B、苷E的回收率范围分别是90.4%～96.8%和87.7%～93.3%;刺五加注射液中刺五加苷B、苷E的回收率范围分别是96.4%～99.8%和95.7%～98.5%.线性范围分别是4.45～22.25 μg·mL-1(r=0.999 8)和5.11～25.55 μg·mL-1(r=0.999 7).结论该方法节约了清洗色谱系统的时间, 提高了测定的灵敏度,提供了评价刺五加制剂质量的方法.
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