淫羊藿总黄酮对大鼠肝微粒体CYP1A2、CYP3A4和CYP2E1活性的影响

摘要

To assess the potential effect of total flavonoids of epimedium (TFE) on cytochrome P450 and activity of its main isoforms in rat liver microsomes. TFE (300 mg/kg) was administered once daily to male Sprague-Dowley rats by gavage for fifteen days. The total cytochrome P450 content and its main isoforms CYP1A2, CYP3A4 and CYP2E1 activities in rat liver microsomes were detected. The activity of CYP1A2 was measured by fluorometry and the activities of CYP3A4 and CYP2E1 were determined by measuring the amount of methanal and p-aminophenol formed using UV/Vis spectrophotometer, respec- tively. Administration of TFE significantly increased the total CYP450 content and activities of CYP 1A2, CYP3A4 and CYP2E1 in rat liver microsomes, compared with the control group. Partieularly, the activities of CYP1A2 and CYP2E1 were enhanced significantly (P<0.01). TFE induced the increase in total CYP450 content and its main isoforms CYP1A2, CYP3A4 and CYP2EI activities in rat liver microsomes.%评估淫羊藿总黄酮对大鼠肝细胞色素P450及其主要亚型活性的潜在影响.淫羊藿总黄酮以300 mg/kg/d的剂量对SD大鼠进行连续灌胃处理15天,测定肝微粒体中CYP450含量与CYP1A2、CYP3A4和CYP2E1亚型活性,观察淫羊藿总黄酮的效应.CYP1A2的活性用荧光比色法进行测定,CYP3A4和CYP2E1的活性用紫外可见分光光度法测定.淫羊藿总黄酮处理后的大鼠肝脏CYP450含量及CYP1A2、CYP3A4和LICYP2E1亚型活性均明显增高,其中CYP1A2和CYP2E1活性升高显著(P<0.01).淫羊藿总黄酮对大鼠肝脏CYP450及主要亚型CYP1A2、CYP3A4和CYP2E1活性均有诱导效应.