本文使用Pirkle型 Sumichiral OA-2500于性柱,研究了正相和反相色谱直接分离阿折地平对映体的流动相条件.使用正相体系,阿折地平在正己烷-乙醇(90∶10,v/v)流动相条件下获得了很好的分离(分离度Rs达到4.0),而使用推荐的流动相正己烷-乙醇(60∶40,v/v),则可以在更短的13分钟内获得分离(分离度Rs为2.7).使用反相的甲醇和含有0.05 mol/L醋酸铵的甲醇做流动相,仅能获得部分分离.用同样的实验条件研究了氨氯地平和尼莫地平的拆分,并在正相条件下获得了部分分离.%Direct chiral separation of azelnidipine enantiomers with Pirkle-type Sumichiral OA-2500 column was studied by normal phase and reserve phase conditions.By normal phase,azelnidipine enantiomers were well separated with Rs as 4.0 using hexane-ethanol (90∶10,v/v) as mobile phase,and Rs as 2.7 in shorter time (no more than 13 min) using hexane-ethanol (60∶40,v/v) as the recommended mobile phase.They were only partially separated by reverse phase using methanol or methanol containing 0.05 mol/L ammonium acetate.Using same chiral column,the chiral separation of other dihydropyridine calcium antagonist analogues almodipine and nimodipine were also studied and showed partial chiral separation in normal phase.
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