羧基化PEG修饰的磁流体的制备和表征

摘要

To prepare and characterize the ferromagnetic fluid of Fe3O4 modified by carboxyl PEG (FF/carboxyl PEG) for hyperthermia of tumor,the magnetic nanoparticles (NPs) of Fe3O4 were prepared by chemical co-precipitation method,and then modified with carboxyl PEG.The iron content of FFs was determined by spectrophotometric method using o-phenanthroline.The stability of FF/carboxyl PEG was assessed by the sedimentation method.FF/carboxyl PEG was characterized by X-ray diffraction (XRD),transmission electron microscopy (TEM),infrared spectrometry (IR) and vibrating sample magnetometer (VSM).Heating effect of FF/carboxyl PEG was measured in an alternating magnetic field in vitro.The stability of FF/carboxyl PEG was much better than that of unmodified ferromagnetic fluid.FF/carboxyl PEG was proved to be composed of Fe3O4 by both XRD and IR.TEM showed that the ferromagnetic particles were well-dispersed.The average particle size was calculated as 5 nm by XRD.The saturation magnetization and residual magnetization of FF/carboxyl PEG were 47.01 and 3.41 emu/g,respectively.The coercive force was 6.7Oe.The specific absorption rate (SAR) of the FF/carboxyl PEG was 63.0 W/g[Fe].The FF/carboxyl PEG shows the promise for hyperthermia of tumor.%制备和表征肿瘤热疗用羧基化PEG修饰的Fe3O4磁流体.化学共沉淀方法制备四氧化三铁磁性纳米颗粒,然后用羧基化PEG修饰；用邻二氮菲显色法测定磁流体中铁的含量；沉降方法考察了制备的磁流体的稳定性；通过X射线衍射、透射电子显微镜、红外和振动样品磁强计对制备的磁流体进行表征；测定了磁流体在交变磁场作用下的热效应.羧基化PEG修饰的磁流体稳定性明显优于未修饰的；红外图谱和X衍射图谱证明所制备的磁流体样品由Fe3O4组成；透射电镜照片显示磁性粒子分散良好；经X衍射数据计算得磁性粒子的粒径约为5 nm;羧基化PEG修饰磁流体的饱和磁化强度和剩余磁化强度分别为47.01和3.41 emu/g.矫顽力为6.7Oe;磁流体的特征吸收率为63.0 W/g[Fe].羧基化PEG修饰的Fe3O4磁流体有望用于肿瘤热疗.