小白杏杏仁油免疫调节及对S180肉瘤抑制作用研究

摘要

研究新疆小白杏杏仁油对大鼠免疫调节作用及对S180肉瘤抑制作用.采用环磷酰胺诱导腹腔注射的方法建立免疫力低下大鼠模型,设立喂养对照组及体外细胞培养阴性对照组,通过酶联免疫吸附检测及细胞培养观察研究其免疫调节及对S180肉瘤抑制作用.新疆小白杏杏仁油可显著提高免疫力低下大鼠模型的脾脏指数(P＜0.01)、胸腺指数(P＜0.01)及肝脏指数(P＜0.05)；对大鼠血清中IgA、IgM、IgG及IL-12指标的改善程度达到较显著差异水平(P＜0.01),IL-2和TNF-α指标的改善程度呈现显著差异水平(P＜0.05),并对体外培养的S180肉瘤细胞具有明显地抑制作用.%To investigate the immunoregulatory activities and the inhibition effect on SI80 sarcom of xiao - bai apricot almond oil. The rat model of immunosuppression was established by intraperitoneal injection of cyclophospha-mide, and the feeding control groups, including empty control group, model control group and sample test group, and cell vitro - culture groups were set up in this paper. The immunoregulatory activities and the inhibition effect on S180 sarcom of xiao - bai apricot almond oil were studied respectively by enzyme - linked immunosorbent assay and cultured cell observation. Xiao - bai apricot almond oil could increase the spleen index (P <0. 01) , the thymus index (P<0.01) and the liver index(P < 0. 05) ; the expression levels of serum immunoglobulin and immune factors, such as IgA,IgM,IgG and IL - 12 were improved greatly (P <0. 01) , the expression levels of other immune factors, such as IL - 2 and TNF - α, were improved obviously ( P < 0.05 ) , and the growth rate of S180 sarcom was inhibited noticeably by the apricot almond oil sample.