大蒜素对人白血病Jurkat细胞生长和凋亡的影响

摘要

Objective To study the effects of allicin on cell growth and apoptosis in human leukemic Jurkat cell line. Methods Electrophoresis mobility shift assay (EMSA) was used to detect the DNA binding activity of NF-κB before and after allicin treatment. The cell growth and apoptosis in Jurkat cells were detected by MTI assay and flow cytometry (FCM) respectively. Results The DNA binding activity of NF-κB decreased after allicin treatment. The growth of Jurkat cells was suppressed in a dose-and time-dependent manner. Conclusion Cell growth inhibition by allicin is related to apoptosis in Jurkat cells, which may take effects through the NF-κB signal pathway.%目的 探讨大蒜素对人白血病Jurkat细胞生长抑制和诱导凋亡的作用.方法 用凝胶直流法(EMSA)分析大蒜素处理前后Jurkat细胞核中NF-κB的DNA结合活性.MTT比色分析法检测大蒜素对Jurkat细胞生长的抑制作用,流式细胞术分析大蒜素对Jurkat细胞凋亡的影响.结果 大蒜素处理后NF-κB的DNA结合活性降低,各浓度大蒜素均能抑制Jurkat细胞生长,其抑制作用呈时间浓度依赖性.结论 大蒜素对Jurkat细胞生长有抑制作用,其抑制作用与诱导Jurkat细胞凋亡有关,可能是通过NF-κB信号途径起作用.