内毒素耐受对细胞因子的影响

摘要

目的：进一步明确内毒素耐受时炎性相关细胞因子的变化。方法昆明鼠分组处置。建立内毒素（LPS）耐受鼠模型。ELISA方法测定血清肿瘤坏死因子α（TNF⁃α），白细胞介素10（IL⁃10）和白细胞介素17（ IL⁃17）浓度。结果 LPS对照组血清TNF⁃α、IL⁃10和IL⁃17浓度分别为（2261.49±1090.28）pg/mL，（7780.42±2320.04）pg/mL，（27.28±13.76）pg/mL，较健康对照组显著增高。而内毒素耐受组相应细胞因子血清浓度分别为（1174.19±450.91）pg/mL，（1293.26±520.85）pg/mL，（22.26±11.44） pg/mL。地塞米松干预进一步降低了TNF⁃α和IL⁃17的水平，但对IL⁃10水平无显著影响。结论内毒素耐受状态与内毒素炎症状态比较，TNF⁃α和IL⁃10水平显著降低，IL⁃17水平无显著变化。应用地塞米松可进一步降低内毒素耐受状态下的TNF⁃α和IL⁃17水平。%Objective To further determine the inflammation⁃associated cytokine changes during endotoxin(LPS)tolerance. Methods Kun⁃ming mice were divided into groups. The endotoxin tolerance mouse model was established with repeated injection of LPS. Serum levels of tumor ne⁃crosis factor⁃α(TNF⁃α),interleukin⁃10(IL⁃10)and IL⁃17 were determined by ELISA assay. Results The serum concentrations of TNF⁃α,IL⁃10 and IL⁃17 were 2 261.49±1 090.28 pg/mL,7 780.42±2 320.04 pg/mL,and 27.28±13.76 pg/mL in the LPS control group,which were increased significantly compared to the normal healthy control group. The serum concentrations of TNF⁃α,IL⁃10 and IL⁃17 were 1 174.19±450.91 pg/mL, 1 293.26±520.85 pg/mL,and 22.26±11.44 pg/mL in the endotoxin tolerance group. Levels of TNF⁃αand IL⁃17 were further decreased by dexameth⁃asone treatment during LPS tolerance inducing,while the IL⁃10 level did not change significantly. Conclusion Compared to the status of endotoxin inflammation,levels of TNF⁃αand IL⁃10 decreased significantly,while levels of IL⁃17 were not significantly changed in the status of endotoxin toler⁃ance. Dexamethasone could further suppress TNF⁃αand IL⁃17 levels during endotoxin tolerance inducing.