重度子痫前期患者外周血中性粒细胞表面黏附分子LFA-1水平变化的研究

摘要

目的：通过检测重度子痫前期孕妇及正常孕妇外周血中性粒细胞计数及中性粒细胞表面黏附分子淋巴细胞功能相关抗原1（LFA⁃1）的表达水平，探讨中性粒细胞活化与子痫前期发病的关系。方法选择2013年11月至2014年2月于中国医科大学附属盛京医院产科就诊的孕产妇28例，分为重度子痫前期组（n=14）和对照组（同期正常妊娠妇女，n=14）。2组孕产妇年龄、孕周无统计学差异。采用流式细胞术检测2组孕产妇外周血中性粒细胞表面黏附分子LFA⁃1的表达情况。检测重度子痫前期组患者平均动脉压，对其与中性粒细胞表面LFA⁃1表达水平进行相关性分析。结果重度子痫前期组外周血中性粒细胞计数[（8.40±2.23）×109/L]明显高于正常妊娠组[（6.71±1.58）×109/L]，差异有统计学意义（P<0.05）。重度子痫前期组外周血中性粒细胞表面黏附分子LFA⁃1表达阳性率[（63.25±38.025）%]明显高于正常妊娠组[（38.32±38.65）%]，差异有统计学意义（P<0.05）。重度子痫前期患者外周血中性粒细胞表面LFA⁃1表达水平与平均动脉压呈显著正相关（r=0.64，P=0.013）。结论重度子痫前期患者外周血中性粒细胞计数及其表面黏附分子LFA⁃1表达均较正常妊娠妇女明显升高，且与病情严重程度显著相关，提示中性粒细胞的活化参与了子痫前期发病。%Objective To check the neutrophil count and the expression of cell surface adhesion molecule,lymphocyte function associated antigen⁃1(LFA⁃1)in peripheral neutrophils in severe preeclamptic patients and normal pregnant women in order to investigate the correlation of neutrophil activation with preeclampsia. Methods Totally 28 pregnant women in the department of gynecology and obstetrics in Shengjing Hospital from No⁃vember 2013 to January 2014 were included in the study,and divided into the severe preeclampsia group(n=14),and the control group(normal pregnant women,n=14). There was no statistically significant difference in age and gestational weeks between the two groups. The expression of LFA⁃1 in peripheral neutrophils was detected by flow cytometry in the two groups. Mean blood pressure(MBP)was measured in the severe preeclamptic group,and the correlation of MBP with expression of LFA⁃1 was analyzed. Results The neutrophil count was 8.40±2.23 ×109/L in the severe pre⁃eclamptic group,significantly higher than(6.71±1.58)×109/L in the control group,(P<0.05). The positive rate of LFA⁃1 in peripheral neutrophils was 63.25±38.025%in the severe preeclamptic patients,significantly higher than 8.32±38.65%in the control group(P<0.05). The expression lev⁃el of LFA⁃1 in peripheral neutrophils was significantly correlated with mean blood pressure in severe preeclamptic patients(r=0.64,P=0.013). Conclusion The neutrophil count and the expression of LFA⁃1 in peripheral neutrophils were significantly increased in severe preeclamptic pa⁃tients compared to normal pregnant women,and significantly correlated with severity of preeclampsia,suggesting that neutrophil activation participat⁃ed in the pathogenesis of preeclampsia.